Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS

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	Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS
	Wang, Tiehui 1; Gao, Qian 1,2; Nie, Pin 2; Secombes, Christopher J.1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2010-10-01
卷号	29 期号:4 页码:656-667
关键词	Rainbow trout SOCS family Expression Modulation Bacterial infection
ISSN号	1050-4648
通讯作者	Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
中文摘要	Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly 1:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1 beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence. (C) 2010 Elsevier Ltd. All rights reserved.
英文摘要	Four new members of the SOCS family of molecules in rainbow trout (Oncorhynchus mykiss), CISH and SOCS6, 7 and 9, are described for the first time in this species. The genes had a wide tissue distribution in trout, and were detected in gills, skin, muscle, liver, spleen, head kidney, intestine and brain, with brain having the highest expression levels. Stimulation of a rainbow trout leucocyte cell line, RTS-11, (mononuclear/macrophage-like cells) with LPS or Poly 1:C had no effect on the expression of these genes, although in both cases the previously identified SOCS1-3 genes were up-regulated. Similarly, stimulation of RTS-11 or RTG-2 (fibroblasts) cells with the trout recombinant cytokines IFN-gamma or IL-1 beta had no effect on CISH or SOCS6, 7 and 9 expression. However, PMA stimulation did impact on SOCS6 and SOCS9 expression, and LPS stimulation of primary cultures or bacterial infection (Yersinia ruckeri) increased significantly CISH expression (as well as SOCS1 and SOCS2 or SOCS3 respectively). It is apparent that the type II SOCS genes (CISH, SOCS1-3) are particularly relevant to immune regulation in fish, although the intriguing expansion of the SOCS4/5 subgroup in fish requires further investigation as to their role and functional divergence. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	GROWTH-FACTOR-BETA ; FUNCTIONAL-CHARACTERIZATION ; MOLECULAR-CLONING ; FUGU-RUBRIPES ; TELEOST FISH ; JAPANESE PUFFERFISH ; IFN-GAMMA ; HOMOLOG ; GENE ; FAMILY
收录类别	SCI
资助信息	EC [513692, Q5RS-2001-002211]; Royal Society
语种	英语
WOS记录号	WOS:000281500500015
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13593]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Wang, Tiehui,Gao, Qian,Nie, Pin,et al. Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(4):656-667.
APA	Wang, Tiehui,Gao, Qian,Nie, Pin,&Secombes, Christopher J..(2010).Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS.FISH & SHELLFISH IMMUNOLOGY,29(4),656-667.
MLA	Wang, Tiehui,et al."Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS".FISH & SHELLFISH IMMUNOLOGY 29.4(2010):656-667.