Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China

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	Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China
	Liu, Jin Yu 1,2; Li, Ai Hua 1; Zhou, Dong Ren 3; Wen, Zhou Rui 4; Ye, Xue Ping 3
刊名	AQUACULTURE RESEARCH
	2010-11-01
卷号	41 期号:12 页码:1835-1844
关键词	isolation characterization Edwardsiella ictaluri yellow catfish
ISSN号	1355-557X
通讯作者	Li, AH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Yellow catfish Pelteobagrus fulvidraco (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well-known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a 'hole in the head', and haemorrhage on the opercula, in the skin under the jaw, creating a 'hole under the jaw'. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. The results showed that yellow catfish isolates were quite conservative pheno-typically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.
英文摘要	Yellow catfish Pelteobagrus fulvidraco (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well-known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a 'hole in the head', and haemorrhage on the opercula, in the skin under the jaw, creating a 'hole under the jaw'. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. The results showed that yellow catfish isolates were quite conservative pheno-typically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.
学科主题	Fisheries
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	CHANNEL CATFISH ; ANTIMICROBIAL SUSCEPTIBILITY ; PUNCTATUS RAFINESQUE ; ENTERIC SEPTICEMIA ; SEQUENCE ALIGNMENT ; PLASMID ANALYSIS ; DANIO-DEVARIO ; E-HOSHINAE ; TARDA ; IDENTIFICATION
收录类别	SCI
资助信息	National Basic Research Program of China (973 Program) [2009CB118705]; Open Fund of Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals
语种	英语
WOS记录号	WOS:000283946200012
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13571]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Zhejiang Inst Freshwater Fisheries, Huzhou, Zhejiang, Peoples R China 4.Hubei Fisheries Sci Res Inst, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jin Yu,Li, Ai Hua,Zhou, Dong Ren,et al. Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China[J]. AQUACULTURE RESEARCH,2010,41(12):1835-1844.
APA	Liu, Jin Yu,Li, Ai Hua,Zhou, Dong Ren,Wen, Zhou Rui,&Ye, Xue Ping.(2010).Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China.AQUACULTURE RESEARCH,41(12),1835-1844.
MLA	Liu, Jin Yu,et al."Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China".AQUACULTURE RESEARCH 41.12(2010):1835-1844.