Responses of Synechocystis sp PCC 6803 (cyanobacterium) photosystem II to pyrene stress

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	Responses of Synechocystis sp PCC 6803 (cyanobacterium) photosystem II to pyrene stress
	Shao, Jihai 1,2,3; Yu, Gongliang 1; Wu, Zhongxing 1; Peng, Xin 1; Li, Renhui 1
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2010
卷号	22 期号:7 页码:1091-1095
关键词	pyrene Synechocystis toxicity photosystem II
ISSN号	1001-0742
通讯作者	Li, RH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	In order to explore the mechanism of acute toxicity for pyrene to cyanobacterial organisms, the responses of Synechocystis sp. PCC 6803 photosystem II (PS II) under pyrene stress were studied. The results showed there was no significant difference about the oxygen evolution under 0.125 mg/L pyrene stress when compared with control, but it was significantly lower than control at 0.625 mg/L pyrene. Polyphasic chlorophyll-a fluorescence transients in cells of Synechocystis sp. PCC 6803 exhibited a typical increase including O, J, I, and P phases. Fluorescence yield at phases J, I and P declined slightly at 0.125 and 0.625 mg/L pyrene, and significantly lower than control at 3.125 mg/L. According to the parameters deviated from JIP-test, no modification was induced by pyrene both at the donor side and at the acceptor side of PS II, and the reaction centre of PS II is the primary damaging target. Based on the expressing of four key genes (psbA, psbB, psbC and psbO) of PS II, only psbA showed significant difference at 3.125 mg/L pyrene when compared with control.
英文摘要	In order to explore the mechanism of acute toxicity for pyrene to cyanobacterial organisms, the responses of Synechocystis sp. PCC 6803 photosystem II (PS II) under pyrene stress were studied. The results showed there was no significant difference about the oxygen evolution under 0.125 mg/L pyrene stress when compared with control, but it was significantly lower than control at 0.625 mg/L pyrene. Polyphasic chlorophyll-a fluorescence transients in cells of Synechocystis sp. PCC 6803 exhibited a typical increase including O, J, I, and P phases. Fluorescence yield at phases J, I and P declined slightly at 0.125 and 0.625 mg/L pyrene, and significantly lower than control at 3.125 mg/L. According to the parameters deviated from JIP-test, no modification was induced by pyrene both at the donor side and at the acceptor side of PS II, and the reaction centre of PS II is the primary damaging target. Based on the expressing of four key genes (psbA, psbB, psbC and psbO) of PS II, only psbA showed significant difference at 3.125 mg/L pyrene when compared with control.
学科主题	Environmental Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	CHLOROPHYLL-A FLUORESCENCE ; SPIRULINA-PLATENSIS ; PSII PHOTOCHEMISTRY ; OXIDATIVE STRESS ; GENE-EXPRESSION ; DROUGHT STRESS ; PSBA GENES ; D1 PROTEIN ; PLANTS ; BIOMARKER
收录类别	SCI
资助信息	National Key Science and Technology Program of China [2008ZX07105-006]; National Natural Science Foundation of China [30821140542]; Chinese Academy of Sciences [0823031501]
语种	英语
WOS记录号	WOS:000280163800019
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13783]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Shao, Jihai,Yu, Gongliang,Wu, Zhongxing,et al. Responses of Synechocystis sp PCC 6803 (cyanobacterium) photosystem II to pyrene stress[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2010,22(7):1091-1095.
APA	Shao, Jihai,Yu, Gongliang,Wu, Zhongxing,Peng, Xin,&Li, Renhui.(2010).Responses of Synechocystis sp PCC 6803 (cyanobacterium) photosystem II to pyrene stress.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,22(7),1091-1095.
MLA	Shao, Jihai,et al."Responses of Synechocystis sp PCC 6803 (cyanobacterium) photosystem II to pyrene stress".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 22.7(2010):1091-1095.