Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 藻类生物学及应用研究中心 > 期刊论文

	Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry
	Gan NanQin; Huang Qun; Zheng LingLing; Song LiRong
刊名	SCIENCE CHINA-LIFE SCIENCES
	2010-08-01
卷号	53 期号:8 页码:973-980
关键词	cyanobacteria bloom toxic-microcystis fluorescence in situ hybridization (FISH) flow cytometry (FCM)
ISSN号	1674-7305
通讯作者	Song, LR, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Toxic cyanobacterial blooms constitute a threat to human safety because Microcystis sp. releases microcystins during growth, and particularly during cell death. Therefore, analysis of toxic and nontoxic Microcystis in natural communities is required in order to assess and predict bloom dynamics and toxin production by these organisms. In this study, an analysis combining fluorescence in situ hybridization (FISH) with flow cytometry (FCM) was used to discriminate between toxic and nontoxic Microcystis and also to quantify the percentage of toxic Microcystis present in blooms. The results demonstrate that the combination of FISH and flow cytometry is a useful approach for studying the ecology of Microcystis toxin production and for providing an early warning for toxic Microcystis blooms.
英文摘要	Toxic cyanobacterial blooms constitute a threat to human safety because Microcystis sp. releases microcystins during growth, and particularly during cell death. Therefore, analysis of toxic and nontoxic Microcystis in natural communities is required in order to assess and predict bloom dynamics and toxin production by these organisms. In this study, an analysis combining fluorescence in situ hybridization (FISH) with flow cytometry (FCM) was used to discriminate between toxic and nontoxic Microcystis and also to quantify the percentage of toxic Microcystis present in blooms. The results demonstrate that the combination of FISH and flow cytometry is a useful approach for studying the ecology of Microcystis toxin production and for providing an early warning for toxic Microcystis blooms.
学科主题	Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	REAL-TIME PCR ; TARGETED OLIGONUCLEOTIDE PROBES ; STRAINS ; QUANTIFICATION ; IMMUNOASSAY ; AERUGINOSA ; SAMPLES ; 16S ; POPULATION ; BACTERIA
收录类别	SCI
资助信息	National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]
语种	英语
WOS记录号	WOS:000281670300008
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13775]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Gan NanQin,Huang Qun,Zheng LingLing,et al. Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(8):973-980.
APA	Gan NanQin,Huang Qun,Zheng LingLing,&Song LiRong.(2010).Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry.SCIENCE CHINA-LIFE SCIENCES,53(8),973-980.
MLA	Gan NanQin,et al."Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry".SCIENCE CHINA-LIFE SCIENCES 53.8(2010):973-980.