Isolation and characterization of microsatellite loci in Rhinogobio ventralis
Xu, Nian1,2,3,4; Shi, Fang1,2,3,4; Xiong, Meihua1,2; Que, Yanfu1,2; Yang, Zhong1,2; Zhu, Bin1,2; Chang, Jianbo1,2
刊名CONSERVATION GENETICS RESOURCES
2010-09-01
卷号2页码:1-3
关键词Rhinogobio ventralis Microsatellites Polymorphic loci Population genetics
ISSN号1877-7252
通讯作者Chang, JB, Minist Water Resources, Inst Hydroecol, 578th Xiongchu Ave, Wuhan 430079, Hubei, Peoples R China ; jbchang@mail.ihe.ac.cn
中文摘要From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.
英文摘要From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]Biodiversity & Conservation ; Genetics & Heredity
关键词[WOS]DNA
收录类别SCI
资助信息hydro-biodiversity monitoring at Pengshui hydropower station on Wujiang River [PS/HB011-2006]
语种英语
WOS记录号WOS:000290413000001
公开日期2011-06-29
内容类型期刊论文
源URL[http://ir.ihb.ac.cn/handle/342005/15576]  
专题水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
作者单位1.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan 430079, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Xu, Nian,Shi, Fang,Xiong, Meihua,et al. Isolation and characterization of microsatellite loci in Rhinogobio ventralis[J]. CONSERVATION GENETICS RESOURCES,2010,2:1-3.
APA Xu, Nian.,Shi, Fang.,Xiong, Meihua.,Que, Yanfu.,Yang, Zhong.,...&Chang, Jianbo.(2010).Isolation and characterization of microsatellite loci in Rhinogobio ventralis.CONSERVATION GENETICS RESOURCES,2,1-3.
MLA Xu, Nian,et al."Isolation and characterization of microsatellite loci in Rhinogobio ventralis".CONSERVATION GENETICS RESOURCES 2(2010):1-3.
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