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	Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage
	Xiong, Daowen1,2; Fang, Tao1; Yu, Linpeng1,2; Sima, Xiaofeng1,2; Zhu, Wentao1,2
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2011-03-15
卷号	409期号:8页码:1444-1452
关键词	Eco-toxicity Metal oxide nanoparticle TiO2 ZnO ROS Oxidative damage
ISSN号	0048-9697
通讯作者	Fang, T, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO2 NPs (96-h LC50 of 124.5 mg/L) to zebrafish was greater than that of the bulk TiO2, which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn2+ solution were quite similar to each other (96-h LC50 of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs,center dot OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of center dot OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of center dot OH. In contrast, exposure to 5 mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50 mg/L TiO2 NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5 mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms. (C) 2011 Elsevier B.V. All rights reserved.
英文摘要	The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO2 NPs (96-h LC50 of 124.5 mg/L) to zebrafish was greater than that of the bulk TiO2, which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn2+ solution were quite similar to each other (96-h LC50 of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs,center dot OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of center dot OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of center dot OH. In contrast, exposure to 5 mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50 mg/L TiO2 NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5 mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Environmental Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	TITANIUM-DIOXIDE NANOPARTICLES ; PERSONAL CARE PRODUCTS ; DAPHNIA-MAGNA ; PSEUDOKIRCHNERIELLA-SUBCAPITATA ; PHOTOCATALYTIC DEGRADATION ; ULTRAFINE PARTICLES ; ONCORHYNCHUS-MYKISS ; CARBON NANOTUBES ; NANOSCALE TIO2 ; FULLERENE C-60
收录类别	SCI
资助信息	Key Project of the National Science and Technology Program [2008ZX07103001]; National Natural Science Foundation of China [21037004]
语种	英语
WOS记录号	WOS:000288634300010
公开日期	2011-06-30
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15654]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Daowen,Fang, Tao,Yu, Linpeng,et al. Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2011,409(8):1444-1452.
APA	Xiong, Daowen,Fang, Tao,Yu, Linpeng,Sima, Xiaofeng,&Zhu, Wentao.(2011).Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage.SCIENCE OF THE TOTAL ENVIRONMENT,409(8),1444-1452.
MLA	Xiong, Daowen,et al."Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage".SCIENCE OF THE TOTAL ENVIRONMENT 409.8(2011):1444-1452.

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