A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater

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	A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater
	Tu, Xinhai 2; Xiao, Bangding 1; Xiong, Jian 2; Chen, Xudong
刊名	TALANTA
	2010-08-15
卷号	82 期号:3 页码:976-983
关键词	Nitrate Nitrite Microplate Water analysis
ISSN号	0039-9140
通讯作者	Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
中文摘要	A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L-1 for NO2--N and 0.02 to 1.50 mg L-1 for NO3--N. The detection limits for nitrite and nitrate were 2 and 4 mu g L-1, respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved.
英文摘要	A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L(-1) for NO(2)(-)-N and 0.02 to 1.50 mg L(-1) for NO(3)(-)-N. The detection limits for nitrite and nitrate were 2 and 4 mu g L(-1), respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Chemistry, Analytical
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	FLOW-INJECTION ANALYSIS ; SPECTROPHOTOMETRIC DETERMINATION ; CADMIUM COLUMN ; COPPER-CADMIUM ; REDUCTION ; PHOSPHATE ; SYSTEM ; SOIL
收录类别	SCI
资助信息	Special Program Water Pollution Control in China [2008ZX07102-005]; Science Foundation of NSFC-Yunnan Province [U0833604]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]
语种	英语
WOS记录号	WOS:000281177100017
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13687]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Tu, Xinhai,Xiao, Bangding,Xiong, Jian,et al. A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater[J]. TALANTA,2010,82(3):976-983.
APA	Tu, Xinhai,Xiao, Bangding,Xiong, Jian,&Chen, Xudong.(2010).A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater.TALANTA,82(3),976-983.
MLA	Tu, Xinhai,et al."A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater".TALANTA 82.3(2010):976-983.