Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China
	Lin, Shen ; Shen, Jianzhong ; Liu, Yang ; Wu, Xingqiang ; Liu, Qigen ; Li, Renhui
刊名	ENVIRONMENTAL MONITORING AND ASSESSMENT
	2011
卷号	175 期号:40547 页码:139-150
关键词	Lake Ulungur Microcystis Real-time PCR HPLC Microcystin Mcy gene Brackish lake
通讯作者	Li, RH, Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; reli@ihb.ac.cn
中文摘要	The distribution and diversity of the waterbloom-forming cyanobacteria, mainly including Microcystis species, were investigated using molecular approaches in Lake Ulungur, an increasingly eutrophic and brackish lake located in Xinjiang, China. Real-time PCR analyses showed the abundance of Microcystis 16S rDNA gene copies in the Ulungur Lake is low, at 7.2 x 10(4) copies L (-aEuro parts per thousand 1) averagely and 4.5 x 10(5) copies L (-aEuro parts per thousand 1) at maximum. Two Microcystis species, M. aeruginosa (Kutzing) Lemmermann and Microcystis wesenbergii (Komarek) Komarek were, for the first time, reported in this lake. The mcyA gene-specific PCR determination on the isolates of Microcystis showed that the M. aeruginosa strains are all mcyA-containing genotypes, while M. wesenbergii are non-mcyA-containing ones. The microcystin contents of the toxic M. aeruginosa strains were shown to be lower than those of the Microcystis strains isolated from other eutrophic lakes in China. Phylogenetic analyses based on 16S rRNA and rpoC1 genes showed that the Microcystis strains isolated from the Ulungur Lake were not genetically divergent from those isolated in the other freshwaters. Such an investigation would contribute to the knowledge on the bloom-forming cyanobacteria of the increasingly eutrophic and saline lakes in the desert area.
学科主题	Environmental Sciences
收录类别	SCI
资助信息	National Basic Research Program of China (973) [2008CB418002]; Chinese Academy of Sciences [082303-1-501]
公开日期	2011-06-30
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15680]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
推荐引用方式 GB/T 7714	Lin, Shen,Shen, Jianzhong,Liu, Yang,et al. Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China[J]. ENVIRONMENTAL MONITORING AND ASSESSMENT,2011,175(40547):139-150.
APA	Lin, Shen,Shen, Jianzhong,Liu, Yang,Wu, Xingqiang,Liu, Qigen,&Li, Renhui.(2011).Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China.ENVIRONMENTAL MONITORING AND ASSESSMENT,175(40547),139-150.
MLA	Lin, Shen,et al."Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China".ENVIRONMENTAL MONITORING AND ASSESSMENT 175.40547(2011):139-150.