Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry

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	Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry
	Ying PQ(应佩青); Yu Y(于泳); Jin G(靳刚); Tao ZL(陶祖莱)
刊名	Colloids and Surfaces B-Biointerfaces
	2003
卷号	32 期号:1 页码:1-10
ISSN号	0927-7765
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
中文摘要	The adsorption and competitive adsorption of collagen and bovine serum albumin (BSA) were directly visualized and quantified using atomic force microscopy (AFM) and imaging ellipsometry. Chemically modified silicon surfaces were used as hydrophilic and hydrophobic substrates. The results showed that collagen and BSA in single component solution adsorbed onto a hydrophobic surface two times more than that onto a hydrophilic surface. The competitive adsorption between collagen and BSA showed that serum albumin preferentially adsorbed onto a hydrophobic surface, while collagen on a hydrophilic surface. In the binary solution of BSA (1 mg/ml BSA) and collagen (0.1 mg/ml), nearly 100% of the protein adsorbed onto the hydrophobic surface was BSA, but on the hydrophilic surface only about 6% was BSA. Surface affinity was the main factor controlling the competitive adsorption.
学科主题	力学
类目[WOS]	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
研究领域[WOS]	Biophysics ; Chemistry ; Materials Science
关键词[WOS]	EPITHELIAL-CELLS ; SERUM-ALBUMIN ; SOLUTION AIR ; COLLAGEN ; INTERFACES ; SURFACES ; ADHESION ; CULTURE
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000185498800001
公开日期	2007-06-15
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/17322]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Ying PQ,Yu Y,Jin G,et al. Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry[J]. Colloids and Surfaces B-Biointerfaces,2003,32(1):1-10.
APA	应佩青,于泳,靳刚,&陶祖莱.(2003).Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry.Colloids and Surfaces B-Biointerfaces,32(1),1-10.
MLA	应佩青,et al."Competitive protein adsorption studied with atomic force microscopy and imaging ellipsometry".Colloids and Surfaces B-Biointerfaces 32.1(2003):1-10.