Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption

	Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption
	Wu, DP ; Zhao, BX ; Dai, ZP ; Qin, JH ; Lin, BC
刊名	lab on a chip
	2006
卷号	6 期号:7 页码:942-947
通讯作者	林炳承
产权排序	1;1
英文摘要	in order to achieve a simple covalent hydrophilic polymer coating on poly(dimethylsiloxane) (pdms) microfluidic chip, epoxy modified hydrophilic polymers were synthesized in aqueous solution with a persulfate radical initiation system, and crosslinked onto pdms pretreated by oxygen plasma and silanized with 3-aminopropyl-triethoxysilanes (aptes). glycidyl methacrylate (gma) was copolymerized with acrylamide (poly(aam-co-gma)) or dimethylacrylamide (poly(dam-co-gma)), and graft polymerized with polyvinylpyrrolidone (pvp-g-gma) or polyvinylalcohol (pva-g-gma). the epoxy groups in the polymers were determined by uv spectra after derivation with benzylamine. reflection absorption infrared spectroscopy (rairs) confirmed covalent grafting of gma-modified polymers onto pdms surface. electroosmotic flow (eof) in the polymer grafted microchannel was strongly suppressed within the range ph 3-11. surface adsorption of lysozyme and bovine serum albumin (bsa) was reduced to less than 10% relative to that on the native pdms surface. on the gma-modified polymer coated pdms microchip, basic proteins, peptides, and sodium dodecyl sulfate (sds) denatured proteins were separated successfully.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology
研究领域[WOS]	biochemistry & molecular biology ; chemistry ; science & technology - other topics
关键词[WOS]	capillary-zone-electrophoresis ; electroosmotic flow ; surface modification ; high-resolution ; devices ; dna ; separation ; channels ; polymerization ; microchips
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000238580900016
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/93875]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China 3.Dalian Med Univ, Coll Lab Med, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wu, DP,Zhao, BX,Dai, ZP,et al. Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption[J]. lab on a chip,2006,6(7):942-947.
APA	Wu, DP,Zhao, BX,Dai, ZP,Qin, JH,&Lin, BC.(2006).Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption.lab on a chip,6(7),942-947.
MLA	Wu, DP,et al."Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption".lab on a chip 6.7(2006):942-947.