Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption | |
Wu, DP; Zhao, BX; Dai, ZP; Qin, JH; Lin, BC | |
刊名 | lab on a chip
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2006 | |
卷号 | 6期号:7页码:942-947 |
通讯作者 | 林炳承 |
产权排序 | 1;1 |
英文摘要 | in order to achieve a simple covalent hydrophilic polymer coating on poly(dimethylsiloxane) (pdms) microfluidic chip, epoxy modified hydrophilic polymers were synthesized in aqueous solution with a persulfate radical initiation system, and crosslinked onto pdms pretreated by oxygen plasma and silanized with 3-aminopropyl-triethoxysilanes (aptes). glycidyl methacrylate (gma) was copolymerized with acrylamide (poly(aam-co-gma)) or dimethylacrylamide (poly(dam-co-gma)), and graft polymerized with polyvinylpyrrolidone (pvp-g-gma) or polyvinylalcohol (pva-g-gma). the epoxy groups in the polymers were determined by uv spectra after derivation with benzylamine. reflection absorption infrared spectroscopy (rairs) confirmed covalent grafting of gma-modified polymers onto pdms surface. electroosmotic flow (eof) in the polymer grafted microchannel was strongly suppressed within the range ph 3-11. surface adsorption of lysozyme and bovine serum albumin (bsa) was reduced to less than 10% relative to that on the native pdms surface. on the gma-modified polymer coated pdms microchip, basic proteins, peptides, and sodium dodecyl sulfate (sds) denatured proteins were separated successfully. |
WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
类目[WOS] | biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology |
研究领域[WOS] | biochemistry & molecular biology ; chemistry ; science & technology - other topics |
关键词[WOS] | capillary-zone-electrophoresis ; electroosmotic flow ; surface modification ; high-resolution ; devices ; dna ; separation ; channels ; polymerization ; microchips |
收录类别 | SCI |
原文出处 | true |
语种 | 英语 |
WOS记录号 | WOS:000238580900016 |
公开日期 | 2010-11-30 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/93875] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China 3.Dalian Med Univ, Coll Lab Med, Dalian, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, DP,Zhao, BX,Dai, ZP,et al. Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption[J]. lab on a chip,2006,6(7):942-947. |
APA | Wu, DP,Zhao, BX,Dai, ZP,Qin, JH,&Lin, BC.(2006).Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption.lab on a chip,6(7),942-947. |
MLA | Wu, DP,et al."Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption".lab on a chip 6.7(2006):942-947. |
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