Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis

	Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
	Chen, XG; Hu, LH; Su, XY; Kong, L; Ye, ML; Zou, HF
刊名	journal of pharmaceutical and biomedical analysis
	2006-02-24
卷号	40 期号:3 页码:559-570
关键词	Honeysuckle ion-exchange chromatography fractionation LC-APCI/MS MALDI-TOF/MS integrated approach
通讯作者	邹汉法
产权排序	1;1
英文摘要	a hyphenated method for the isolation and identification of components in a traditional chinese medicine of honeysuckle was developed. ion-exchange chromatography (iec) was chosen for the fractionation of honeysuckle extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. each of the enriched fractions was then further analyzed by reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer (rplc-apci/ms) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof/ms) with matrix of oxidized carbon nanotubes, respectively. it can be noted totally more than 117 components were detected by uv detector, apci/ms and maldi-tof/ms in honeysuckle extract except the, 145 components identified by maldi-tof/ms alone with this integrated approach, and 7 of them were preliminary identified according to their uv spectra and mass spectra performed by apci/ms and maldi-tof/ms, respectively. the obtained analytical results not only indicated the approach of integration iec fractionation with rplc-apci/ms and maldi-tof/ms is capable of analyzing complex samples, but also exhibited the potential power of the mass spectrometer in detection of low-mass compounds, such as traditional chinese medicines (tcms) and complex biological samples. (c) 2005 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	counter-current chromatography ; traditional chinese medicines ; artemisia-capillaris ; active constituents ; lonicera-japonica ; carbon nanotubes ; chlorogenic acid ; flos-lonicerae ; identification ; resolution
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000235821100010
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/93379]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Chen, XG,Hu, LH,Su, XY,et al. Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis[J]. journal of pharmaceutical and biomedical analysis,2006,40(3):559-570.
APA	Chen, XG,Hu, LH,Su, XY,Kong, L,Ye, ML,&Zou, HF.(2006).Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis.journal of pharmaceutical and biomedical analysis,40(3),559-570.
MLA	Chen, XG,et al."Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis".journal of pharmaceutical and biomedical analysis 40.3(2006):559-570.