Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography

	Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography
	Zhang, Y; Chen, JP; Zhang, CZ; Wu, WZ; Liang, XM
刊名	journal of steroid biochemistry and molecular biology
	2005-03-01
卷号	94 期号:4 页码:375-381
关键词	phytoestrogens kudzu root recombinant yeast HPLC
通讯作者	梁鑫淼
产权排序	1;1
英文摘要	the estrogenic activity of the chinese herb kudzu root was investigated by a recombinant yeast screening assay (yes). isoflavones are the main components in the plant, of which puerarin is the most abundant one. the kudzu root extract was separated into four fractions according to the polarity. the crude extract and its sub-fractions, except the water fraction, showed clear estrogenic activity and the potencies were in the range of 10(-3) to 10(-1) g/l. the ligand potency was used to compare the estrogenic activity of these fractions. the crude extract and its sub-fractions were further analyzed by high performance liquid chromatography (hplc) to correlate the activity and the active components. bioassay and chemical analysis showed that theoretical estrogenic activity expressed as equivalent 17 beta-estradiol concentration or the cumulative effects are comparable to that experimentally determined by yes. the results showed that the high content of isoflavones as well as the high estrogenic activity could make kudzu root extract an interesting candidate for hormone replacement therapy. (c) 2005 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; endocrinology & metabolism
研究领域[WOS]	biochemistry & molecular biology ; endocrinology & metabolism
关键词[WOS]	hormone replacement therapy ; large-scale use ; isoflavones ; flavonoids ; puerariae ; genistein ; daidzein ; extracts ; radix ; risk
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000229445800011
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/92509]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Y,Chen, JP,Zhang, CZ,et al. Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography[J]. journal of steroid biochemistry and molecular biology,2005,94(4):375-381.
APA	Zhang, Y,Chen, JP,Zhang, CZ,Wu, WZ,&Liang, XM.(2005).Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography.journal of steroid biochemistry and molecular biology,94(4),375-381.
MLA	Zhang, Y,et al."Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography".journal of steroid biochemistry and molecular biology 94.4(2005):375-381.