Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification

	Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification
	Jin, WH ; Dai, J ; Li, SJ ; Xia, QC ; Zou, HF ; Zeng, R
刊名	journal of proteome research
	2005-03-01
卷号	4 期号:2 页码:613-619
关键词	proteomics human plasma mass spectrometry two-dimensional liquid chromatography protein fractionation
ISSN号	1535-3893
通讯作者	邹汉法 ; 曾嵘
产权排序	2;1
英文摘要	a resurgence of interest in the human plasma proteome has occurred in recent years because it holds great promise of revolution in disease diagnosis and therapeutic monitoring. as one of the most powerful separation techniques, multidimensional liquid chromatography has attracted extensive attention, but most published works have focused on the fractionation of tryptic peptides. in this study, proteins from human plasma were prefractionated by online sequential strong cation exchange chromatography and reversed-phase chromatography. the resulting 30 samples were individually digested by trypsin, and analyzed by capillary reversed-phase liquid chromatography coupled with linear ion trap mass spectrometry. after meeting stringent criteria, a total of 1292 distinct proteins were successfully identified in our work, among which, some proteins known to be present in serum in < 10 ng/ml were detected. compared with other works in published literatures, this analysis offered a more full-scale list of the plasma proteome. considering our strategy allows high throughput of protein identification in serum, the prefractionation of proteins before ms analysis is a simple and effective method to facilitate human plasma proteome research.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	resolution 2-dimensional electrophoresis ; human serum proteome ; hepatocellular-carcinoma ; cancer-diagnosis ; ovarian-cancer ; proteins ; biomarkers ; population ; system ; gels
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000228421900049
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/92177]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Inst Biochem & Cell Biol,Key Lab Proteom, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Jin, WH,Dai, J,Li, SJ,et al. Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification[J]. journal of proteome research,2005,4(2):613-619.
APA	Jin, WH,Dai, J,Li, SJ,Xia, QC,Zou, HF,&Zeng, R.(2005).Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification.journal of proteome research,4(2),613-619.
MLA	Jin, WH,et al."Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification".journal of proteome research 4.2(2005):613-619.