Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA

	Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA
	Zhou, XM ; Dai, ZP ; Liu, X ; Luo, Y ; Wang, H ; Lin, BC
刊名	journal of separation science
	2005-02-01
卷号	28 期号:3 页码:225-233
关键词	DNA separation electrophoretic microchip fabrication modification
ISSN号	1615-9306
通讯作者	林炳承
产权排序	1;1
英文摘要	inexpensive and permanently modified poly(methyl methacrylate)(pmma) microchips were fabricated by an injection-molding process. a novel sealing method for plastic microchips at room temperature was introduced. run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values between 1-3% for the run-to-run and less than 2.1% for the chip-to-chip comparisons. acrylonitrile-butadiene-styrene (abs) was used as an additive in pmma substrates. the proportions of pmma and abs were optimized. abs may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro-osmotic flow (eof). the detection limit of rhodamine 6g dye for the modified microchip on the home-made microchip analyzer showed a dramatic 100-fold improvement over that for the unmodified pmma chip. a detection limit of the order of 10(-20) mole has been achieved for each injected phix-174/haeiii dna fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 dna restriction fragments within 180 seconds. analysis of a pcr product from the tobacco act gene was performed on the modified microchip as an application example.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	capillary-electrophoresis ; microfluidic devices ; fabrication ; poly(dimethylsiloxane) ; molecules ; fragments ; flow
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000227397400002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/92149]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China
推荐引用方式 GB/T 7714	Zhou, XM,Dai, ZP,Liu, X,et al. Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA[J]. journal of separation science,2005,28(3):225-233.
APA	Zhou, XM,Dai, ZP,Liu, X,Luo, Y,Wang, H,&Lin, BC.(2005).Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA.journal of separation science,28(3),225-233.
MLA	Zhou, XM,et al."Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA".journal of separation science 28.3(2005):225-233.