Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets

doi:10.1016/j.ijms.2018.09.016

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	Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets
	Guo, Mingquan 1,2; Chen, Guilin 1,2
刊名	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
	2018-11-01
卷号	434 页码:87-92
关键词	Ultrafiltration-LC/MS Polyphyllin Topoisomerase I and II Cyclooxygenase 2 Multiple drug targets
ISSN号	1387-3806
DOI	10.1016/j.ijms.2018.09.016
英文摘要	Paris polyphylla Smith var. yunnanensis (P. polyphylla) has been widely used as a traditional Chinese medicine for centuries. The Chinese Pharmacopoeia (2015) suggests that the four steroidal saponins, namely polyphyllin I, II, VI and VII, are considered to be the marker components for the quality control (QC) of this herb medicine. However, the active components responsible for the anti-cancer and anti-inflammatory effects of P. polyphylla and the underlying mechanisms of action remain elusive. In the present study, ultrafiltration HPLC-MS (UF-LC-MS) with the multiple drug targets of topoisomerase I, II (Top I, II) and cyclooxygenase 2 (COX-2) was carried out to systematically investigate the mechanisms of action in a multi-component manner. The results revealed that the polyphyllin I displayed not only higher binding affinity to the three drug targets, but also stronger inhibitory activities, as compared with the positive controls. Thus, COX-2 and Top I could be considered as the anti-cancer and anti-inflammatory targets of P. polyphylla. Further molecular docking studies partly revealed the inhibitory mechanism of action associated with the hydrogen bonds between polyphyllin I and the three target enzymes. This study showcased the high efficiency of OF-LC/MS in exploring the potential bioactive components from P. polyphylla and its possible mechanisms of action in a multi-target and multi-component manner. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81673580] ; Major Project for Special Technology Innovation of Hubei Province - Sinoforeign Cooperation Project[2017AHB054]
WOS研究方向	Physics ; Spectroscopy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000449709900013
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6164]
专题	中国科学院武汉植物园
通讯作者	Guo, Mingquan
作者单位	1.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Guo, Mingquan,Chen, Guilin. Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2018,434:87-92.
APA	Guo, Mingquan,&Chen, Guilin.(2018).Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,434,87-92.
MLA	Guo, Mingquan,et al."Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 434(2018):87-92.