The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP)

doi:10.1099/jgv.0.000982

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	The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP)
	Lu, Baojing 1; Xie, Li 3,4; Zheng, Zhenhua 3; Miao, Yuanjiu 3; Liu, Yan 3; Zhang, Yuan 3; Zheng, Caishang 3; Ke, Xianliang 3; Hu, Qinxue 2; Wang, Hanzhong 3
刊名	JOURNAL OF GENERAL VIROLOGY
	2018
卷号	99 期号:1 页码:73-85
关键词	3C enterovirus A71 zinc finger antiviral protein cleavage
ISSN号	0022-1317
DOI	10.1099/jgv.0.000982
英文摘要	Enterovirus A71 (EV-A71) is a positive-strand RNA virus that causes hand-foot-mouth disease and neurological complications in children and infants. Although the underlying mechanisms remain to be further defined, impaired immunity is thought to play an important role. The host zinc-finger antiviral protein (ZAP), an IFN-stimulated gene product, has been reported to specifically inhibit the replication of certain viruses. However, whether ZAP restricts the infection of enteroviruses remains unknown. Here, we report that EV-A71 infection upregulates ZAP mRNA in RD and HeLa cells. Moreover, ZAP overexpression rendered 293T cells resistant to EV-A71 infection, whereas siRNA-mediated depletion of endogenous ZAP enhanced EV-A71 infection. The EV-A71 infection stimulated site-specific proteolysis of two ZAP isoforms, leading to the accumulation of a 40 kDa N-terminal ZAP fragment in virus-infected cells. We further revealed that the 3C protease (3Cpro) of EV-A71 mediates ZAP cleavage, which requires protease activity. Furthermore, ZAP variants with single amino acid substitutions at Gln-369 were resistant to 3Cpro cleavage, implying that Gln-369 is the sole cleavage site in ZAP. Moreover, although ZAP overexpression inhibited EV-A71 replication, the cleaved fragments did not show this effect. Our results indicate that an equilibrium between ZAP and enterovirus 3Cpro controls viral infection. The findings in this study suggest that viral 3Cpro mediated ZAP cleavage may represent a mechanism to escape host antiviral responses.
资助项目	National Natural Science Foundation of China (NSFC)[81371811] ; National Natural Science Foundation of China (NSFC)[81401658]
WOS研究方向	Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:000431016500009
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5109]
专题	中国科学院武汉植物园
通讯作者	Hu, Qinxue; Wang, Hanzhong
作者单位	1.Anhui Med Univ, Sch Basic Med Sci, Dept Microbiol, Hefei 230032, Anhui, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lu, Baojing,Xie, Li,Zheng, Zhenhua,et al. The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP)[J]. JOURNAL OF GENERAL VIROLOGY,2018,99(1):73-85.
APA	Lu, Baojing.,Xie, Li.,Zheng, Zhenhua.,Miao, Yuanjiu.,Liu, Yan.,...&Wang, Hanzhong.(2018).The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP).JOURNAL OF GENERAL VIROLOGY,99(1),73-85.
MLA	Lu, Baojing,et al."The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP)".JOURNAL OF GENERAL VIROLOGY 99.1(2018):73-85.