Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)

doi:10.3389/fgene2018.00117

CORC > 武汉植物园 > 中国科学院武汉植物园

	Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)
	Hu, Hongxia 1; Chen, Ji 3; Wang, Wei 1; Tian, Zhaohui 1; Dong, Ying 1; Dong, Tian 1; Zhu, Hua 1; Zhu, Zuoyan 3; Hu, Wei 2,3
刊名	FRONTIERS IN GENETICS
	2018-04-06
卷号	9 页码:9
关键词	sterlet gene transfer gene editing EGFP no tail
ISSN号	1664-8021
DOI	10.3389/fgene2018.00117
英文摘要	The sturgeon (Acipenseriformes) is an important farmed species because of its economical value. However, neither gene transfer nor gene editing techniques have been established in sturgeon for molecular breeding and gene functional study until now. In this study, we accomplished gene transfer and gene editing in sterlet (Acipenser ruthenus), which has the shortest sexual maturation period of sturgeons. The plasmid encoding enhanced green fluorescent protein (EGFP) was transferred into the embryos of sterlet at injection concentration of 100 ng/mu L, under which condition high survival rate and gene transfer rate could be achieved. Subsequently, exogenous EGFP was efficiently disrupted by transcription activator-like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 nuclease/guide RNA (gRNA), with injection concentrations of 300 ng/mu L TALENs, or 100 ng/mu L Cas9 nuclease and 30 ng/mu L gRNA, respectively, under which condition high survival rate and gene mutation rate could be achieved. Finally, the endogenous gene no tail in sterlet was successfully mutated by Cas9 nuclease/gRNA. We observed the CRISPR-induced no tail mutation, at a high efficiency with the mutant P0 embryos displaying the expected phenotype of bent spine and twisted tail.
资助项目	National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31325026] ; Foundation of Beijing Municipal Science and Technology Project[Z161100004516003] ; Innovation Team of Sturgeon and Salmonid of Beijing Project[BAIC08-2018] ; Innovation Team of Sturgeon and Salmonid of Baafs Project[JNKST201611] ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry
WOS研究方向	Genetics & Heredity
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000429371100001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5053]
专题	中国科学院武汉植物园
通讯作者	Hu, Hongxia; Hu, Wei
作者单位	1.Beijing Fisheries Res Inst, Beijing Key Lab Fishery Biotechnol, Beijing, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Hu, Hongxia,Chen, Ji,Wang, Wei,et al. Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)[J]. FRONTIERS IN GENETICS,2018,9:9.
APA	Hu, Hongxia.,Chen, Ji.,Wang, Wei.,Tian, Zhaohui.,Dong, Ying.,...&Hu, Wei.(2018).Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus).FRONTIERS IN GENETICS,9,9.
MLA	Hu, Hongxia,et al."Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)".FRONTIERS IN GENETICS 9(2018):9.