Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence

doi:10.1016/j.neuroimage.2015.10.058

CORC > 武汉植物园 > 中国科学院武汉植物园

	Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence
	Wu, Ruiqi 3,4; Zhou, Xin 3,4; Qiu, Bensheng 1; Lei, Hao 3,4; Zhang, Yaoyu 6,7; Gao, Jia-Hong 2,6,7; Chai, Yuhui 6,7; Bi, Guoqiang 5; Wang, Liping 8; Xu, Fuqiang 3,4
刊名	NEUROIMAGE
	2016-01-15
卷号	125 页码:533-543
关键词	Neuronal current Neuronal electrical activity Oscillatory current Spin-lock Functional MRI Optogenetics
ISSN号	1053-8119
DOI	10.1016/j.neuroimage.2015.10.058
英文摘要	The direct detection of neuronal electrical activity is one of the most challenging goals in non-BOLD fMRI research. Previous work has demonstrated its feasibility in phantom and cell culture studies, but attempts in in vivo studies remain fewand far between. Most recent in vivo studies used T2*-weighted sequences to directly detect neuronal electrical activity evoked by sensory stimulus. As neuronal electrical signal is usually comprised of a series of spectrally distributed oscillatorywaveforms rather than being a direct current, it is most likely to be detected using oscillatory current sensitive sequences. In this study, we explored the potential of using the spin-lock oscillatory excitation (SLOE) sequence with spiral readout to directly detect optogenetically evoked oscillatory neuronal electrical activity, whose main spectral component can be manipulated artificially to match the resonance frequency of spin-lock RF field. In addition, experiments using the stimulus-induced rotary saturation (SIRS) sequence with spiral readout were also performed. Electrophysiological recording and MRI data acquisition were conducted on separate animals. Robust optogenetically evoked oscillatory LFP signals were observed and significant BOLD signals were acquired with the GE-EPI sequence before and after the whole SLOE and SIRS acquisitions, but no significant neuronal current MRI (ncMRI) signal changes were detected. These results indicate that the sensitivity of oscillatory current sensitive sequences needs to be further improved for direct detection of neuronal electrical activity. (C) 2015 Elsevier Inc. All rights reserved.
资助项目	China's National Strategic Basic Research Program (973)[2012CB720700] ; Natural Science Foundation of China[81227003] ; Natural Science Foundation of China[81430037] ; Natural Science Foundation of China[31421003] ; Natural Science Foundation of China[91132307] ; Natural Science Foundation of China[31171061] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02050002]
WOS研究方向	Neurosciences & Neurology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000366647500048
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/4187]
专题	中国科学院武汉植物园
通讯作者	Gao, Jia-Hong
作者单位	1.Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei 230026, Peoples R China 2.Peking Univ, McGovern Inst Brain Res, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Beijing 100864, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China 6.Peking Univ, Ctr MRI Res, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China 7.Peking Univ, Sch Phys, Inst Heavy Ion Phys, Beijing City Key Lab Med Phys & Engn, Beijing 100871, Peoples R China 8.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ruiqi,Zhou, Xin,Qiu, Bensheng,et al. Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence[J]. NEUROIMAGE,2016,125:533-543.
APA	Wu, Ruiqi.,Zhou, Xin.,Qiu, Bensheng.,Lei, Hao.,Zhang, Yaoyu.,...&Xu, Fuqiang.(2016).Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence.NEUROIMAGE,125,533-543.
MLA	Wu, Ruiqi,et al."Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence".NEUROIMAGE 125(2016):533-543.