Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms

doi:10.1038/srep07242

CORC > 武汉植物园 > 中国科学院武汉植物园

	Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms
	Chen, Yongzhi 1,2; Wang, Shanshan ; Yi, Zhaohong 1; Tian, Huabin 1,2; Aliyari, Roghiyh 3; Li, Yanhua 7; Chen, Gang 7; Liu, Ping 1; Zhong, Jin 8; Chen, Xinwen 9
刊名	SCIENTIFIC REPORTS
	2014-12-03
卷号	4 页码:8
ISSN号	2045-2322
DOI	10.1038/srep07242
英文摘要	Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry. However, little is known about the function of CH25H on HCV infection and replication and whether antiviral function of CH25H is exclusively mediated by 25HC. In the present study, we have found that although 25HC produced by CH25H can inhibit HCV replication, CH25H mutants lacking the hydroxylase activity still carry the antiviral activity against HCV but not other viruses such as MHV-68. Further studies have revealed that CH25H can interact with the NS5A protein of HCV and inhibit its dimer formation, which is essential for HCV replication. Thus, our work has uncovered a novel mechanism by which CH25H restricts HCV replication, suggesting that CH25H inhibits viral infection through both 25HC-dependent and independent events.
资助项目	National Natural Science Foundation of China[81325012] ; Ministry of Science and Technology of China[2015CB554302] ; Ministry of Science and Technology of China[2009CB522505] ; Ministry of Science and Technology of China[2013CB911103] ; National Institutes of Health[AI078389] ; National Institutes of Health[AI069120]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000346264000001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/3904]
专题	中国科学院武汉植物园
通讯作者	Qin, F. Xiao-Feng
作者单位	1.Chinese Acad Sci, Inst Biophys, Key Lab Infect & Immun, Beijing 100080, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA 4.Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA 5.Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA 6.Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA 7.CAAS, Inst Qual Standard & Testing Technol Agroproduct, Beijing, Peoples R China 8.Chinese Acad Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, Shanghai, Peoples R China 9.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yongzhi,Wang, Shanshan,Yi, Zhaohong,et al. Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms[J]. SCIENTIFIC REPORTS,2014,4:8.
APA	Chen, Yongzhi.,Wang, Shanshan.,Yi, Zhaohong.,Tian, Huabin.,Aliyari, Roghiyh.,...&Cheng, Genhong.(2014).Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms.SCIENTIFIC REPORTS,4,8.
MLA	Chen, Yongzhi,et al."Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms".SCIENTIFIC REPORTS 4(2014):8.