The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers

doi:10.1104/pp.16.01956

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	The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers
	Yang, Xianpeng 2,5; Zhao, Huayan 4; Kosma, Dylan K.6; Tomasi, Pernell 1; Dyer, John M.1; Li, Rongjun 2; Liu, Xiulin 2,5; Wang, Zhouya 2,5; Parsons, Eugene P.7; Jenks, Matthew A.8
刊名	PLANT PHYSIOLOGY
	2017-02-01
卷号	173 期号:2 页码:1109-1124
ISSN号	0032-0889
DOI	10.1104/pp.16.01956
英文摘要	We report n-6 monounsaturated primary alcohols (C-26:1, C-28:1, and C-30:1 homologs) in the cuticular waxes of Arabidopsis (Arabidopsis thaliana) inflorescence stem, a class of wax not previously reported in Arabidopsis. The Arabidopsis cer17 mutant was completely deficient in these monounsaturated alcohols, and CER17 was found to encode a predicted ACYL-COENZYME A DESATURASE LIKE4 (ADS4). Studies of the Arabidopsis cer4 mutant and yeast variously expressing CER4 (a predicted fatty acyl-CoA reductase) with CER17/ADS4, demonstrated CER4's principal role in synthesis of these monounsaturated alcohols. Besides unsaturated alcohol deficiency, cer17 mutants exhibited a thickened and irregular cuticle ultrastructure and increased amounts of cutin monomers. Although unsaturated alcohols were absent throughout the cer17 stem, the mutation's effects on cutin monomers and cuticle ultrastructure were much more severe in distal than basal stems, consistent with observations that the CER17/ADS4 transcript was much more abundant in distal than basal stems. Furthermore, distal but not basal stems of a double mutant deficient for both CER17/ADS4 and LONG-CHAIN ACYL-COA SYNTHETASE1 produced even more cutin monomers and a thicker and more disorganized cuticle ultrastructure and higher cuticle permeability than observed for wild type or either mutant parent, indicating a dramatic genetic interaction on conversion of very long chain acyl-CoA precursors. These results provide evidence that CER17/ADS4 performs n-6 desaturation of very long chain acyl-CoAs in both distal and basal stems and has a major function associated with governing cutin monomer amounts primarily in the distal segments of the inflorescence stem.
资助项目	Natural Science Foundation of China[31370338] ; Natural Science Foundation of China[31570186]
WOS研究方向	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000394140800015
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1416]
专题	中国科学院武汉植物园
通讯作者	Lu, Shiyou
作者单位	1.ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 4.Wuhan Inst Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA 7.Prairie State Coll, Chicago Hts, IL 60411 USA 8.West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA
推荐引用方式 GB/T 7714	Yang, Xianpeng,Zhao, Huayan,Kosma, Dylan K.,et al. The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers[J]. PLANT PHYSIOLOGY,2017,173(2):1109-1124.
APA	Yang, Xianpeng.,Zhao, Huayan.,Kosma, Dylan K..,Tomasi, Pernell.,Dyer, John M..,...&Lu, Shiyou.(2017).The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers.PLANT PHYSIOLOGY,173(2),1109-1124.
MLA	Yang, Xianpeng,et al."The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers".PLANT PHYSIOLOGY 173.2(2017):1109-1124.