Microsatellite loci transferability in chestnut

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	Microsatellite loci transferability in chestnut
	Wang, Ying 2,3; Kang, Ming 1; Huang, Hongwen 1
刊名	JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
	2008-09-01
卷号	133 期号:5 页码:692-700
关键词	SSR Castanea genetic diversity
ISSN号	0003-1062
英文摘要	Cross-species amplification of 55 microsatellite loci developed in european chestnut (Castanea sativa Mill.) and japanese chestnut (C. erenata Sieb & Zucc.) was tested in three chestnut species from China [C. mollissima Blume, C. seguinii Dode, and C. henryi (Skan.) Rehder & Wilson]. Among all the tested loci, 47 (85.5%), 47 (85.5%), and 44 (80%) were successfully amplified in each of the three Chinese species, respectively. All microsatellite loci tested from C. crenata successfully amplified in the Chinese species, while only 80.5%, 80.5%, and 73.2% of the loci originating from C. sativa amplified in the three Chinese species. The level of polymorphism and mean number of alleles was 58.2% and 4.12 for C mollissima, 60% and 4.64 for C. seguinii, and 60% and 4.76 for C. henryi, with mean observed heterozygosity ranging from 0.440 to 0.549 and mean expected heterozygosity ranging from 0.506 to 0.615. Transferability of Castanea Mill. microsatellites provides a powerful tool for chestnut breeding programs and conservation genetic studies of Castanea species.
资助项目	National Natural Science Foundation of China[30770219] ; Chinese Academy of Science[KSCX2-YW-N-061] ; Laboratory of Biodiversity Conservation and Sustainable Utilization, SCBG-CAS[BCSU 080112]
WOS研究方向	Agriculture
语种	英语
出版者	AMER SOC HORTICULTURAL SCIENCE
WOS记录号	WOS:000259690100009
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1085]
专题	中国科学院武汉植物园
通讯作者	Huang, Hongwen
作者单位	1.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ying,Kang, Ming,Huang, Hongwen. Microsatellite loci transferability in chestnut[J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,2008,133(5):692-700.
APA	Wang, Ying,Kang, Ming,&Huang, Hongwen.(2008).Microsatellite loci transferability in chestnut.JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,133(5),692-700.
MLA	Wang, Ying,et al."Microsatellite loci transferability in chestnut".JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 133.5(2008):692-700.