High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles

doi:10.1111/j.1744-7909.2008.00785.x

CORC > 武汉植物园 > 中国科学院武汉植物园

	High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles
	Chen, Ji-Feng 1,2,4; Li, Ying 3; Wang, Zhen-Fang 3; Li, Ji-Lun 3; Jiang, Wei 3; Li, Shao-Hua 2,4
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2009-04-01
卷号	51 期号:4 页码:409-413
关键词	bacterial magnetic particle detection sensitivity ELISA fluoroimmunoassay grapevine fanleaf virus prunus necrotic ring spot virus
ISSN号	1672-9072
DOI	10.1111/j.1744-7909.2008.00785.x
英文摘要	Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 x 10(6) dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10(6)) higher than an ELISA-based method of detection PNRSV and GFLV.
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000264735600008
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1048]
专题	中国科学院武汉植物园
通讯作者	Li, Shao-Hua
作者单位	1.Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.China Agr Univ, Dept Microbiol & Immunol, Beijing 100094, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Chen, Ji-Feng,Li, Ying,Wang, Zhen-Fang,et al. High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2009,51(4):409-413.
APA	Chen, Ji-Feng,Li, Ying,Wang, Zhen-Fang,Li, Ji-Lun,Jiang, Wei,&Li, Shao-Hua.(2009).High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,51(4),409-413.
MLA	Chen, Ji-Feng,et al."High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 51.4(2009):409-413.