Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow)

doi:10.1016/j.scienta.2010.02.001

CORC > 武汉植物园 > 中国科学院武汉植物园

	Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow)
	Wang, Yong 2; Li, Jing 1; Xia, Renxue 2
刊名	SCIENTIA HORTICULTURAE
	2010-06-03
卷号	125 期号:2 页码:110-116
关键词	Satsuma mandarin Flavonoid Chalcone synthase Chalcone isomerase Real-time PCR HPLC
ISSN号	0304-4238
DOI	10.1016/j.scienta.2010.02.001
英文摘要	Chalcone synthase (CHS) and chalcone isomerase (CHI) are two key genes involved in flavonoid biosynthesis. They were both cloned from Guoqing No. 4 satsuma mandarin (Citrus unshiu marcow), and their relative expression and accumulation of corresponding flavonoid components during fruit maturation were investigated by real-time PCR and HPLC techniques, respectively. During fruit maturation, expression of CHS and CHI genes declined gradually in peels, as well as the concentrations of total flavonoids, trans-chalcone, narirutin and hesperidin; in pulps, however, expression of both genes showed an approximately uptrend, and the concentrations of total flavonoids and those three components were detected in a lower level without significant changes among different developmental periods (P < 0.05). This research confirmed that expression of CHS and CHI genes was positively correlated with flavonoid accumulation and overexpression of them could be a potential approach to produce massive desired flavonoids in citrus fruits. (C) 2010 Published by Elsevier B.V.
资助项目	National Public Benefit (Agriculture) Research Foundation[nyhyzx07-023] ; Ministry of Science and Technology[2004EP090019]
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000278579800007
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/945]
专题	中国科学院武汉植物园
通讯作者	Xia, Renxue
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Subject Grp Mol Breeding Fruit Trees, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yong,Li, Jing,Xia, Renxue. Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow)[J]. SCIENTIA HORTICULTURAE,2010,125(2):110-116.
APA	Wang, Yong,Li, Jing,&Xia, Renxue.(2010).Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow).SCIENTIA HORTICULTURAE,125(2),110-116.
MLA	Wang, Yong,et al."Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow)".SCIENTIA HORTICULTURAE 125.2(2010):110-116.