Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes | |
Chan, Zhulong2; Grumet, Rebecca1; Loescher, Wayne1 | |
刊名 | JOURNAL OF EXPERIMENTAL BOTANY |
2011-10-01 | |
卷号 | 62期号:14页码:4787-4803 |
关键词 | Abscisic acid cell wall mannitol mannose-6-phosphate reductase osmoprotectant redox salt tolerance |
ISSN号 | 0022-0957 |
DOI | 10.1093/jxb/err130 |
英文摘要 | Mannitol is a putative osmoprotectant contributing to salt tolerance in several species. Arabidopsis plants transformed with the mannose-6-phosphate reductase (M6PR) gene from celery were dramatically more salt tolerant (at 100 mM NaCl) as exhibited by reduced salt injury, less inhibition of vegetative growth, and increased seed production relative to the wild type (WT). When treated with 200 mM NaCl, transformants produced no seeds, but did bolt, and exhibited less chlorosis/necrosis and greater survival and dry weights than the WT. Without salt there were no M6PR effects on growth or phenotype, but expression levels of 2272 genes were altered. Many fewer differences (1039) were observed between M6PR and WT plants in the presence of salt, suggesting that M6PR pre-conditioned the plants to stress. Previous work suggested that mannitol is an osmoprotectant, but mannitol levels are invariably quite low, perhaps inadequate for osmoprotectant effects. In this study, transcriptome analysis reveals that the M6PR transgene activated the downstream abscisic acid (ABA) pathway by up-regulation of ABA receptor genes (PYL4, PYL5, and PYL6) and down-regulation of protein phosphatase 2C genes (ABI1 and ABI2). In the M6PR transgenic lines there were also increases in transcripts related to redox and cell wall-strengthening pathways. These data indicate that mannitol-enhanced stress tolerance is due at least in part to increased expression of a variety of stress-inducible genes. |
资助项目 | USDA-BRAG[2005-39454-16516] |
WOS研究方向 | Plant Sciences |
语种 | 英语 |
出版者 | OXFORD UNIV PRESS |
WOS记录号 | WOS:000295983800007 |
内容类型 | 期刊论文 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/841] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Loescher, Wayne |
作者单位 | 1.Michigan State Univ, PSSB, Grad Program Plant Breeding Genet & Biotechnol, E Lansing, MI 48864 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
推荐引用方式 GB/T 7714 | Chan, Zhulong,Grumet, Rebecca,Loescher, Wayne. Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes[J]. JOURNAL OF EXPERIMENTAL BOTANY,2011,62(14):4787-4803. |
APA | Chan, Zhulong,Grumet, Rebecca,&Loescher, Wayne.(2011).Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes.JOURNAL OF EXPERIMENTAL BOTANY,62(14),4787-4803. |
MLA | Chan, Zhulong,et al."Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes".JOURNAL OF EXPERIMENTAL BOTANY 62.14(2011):4787-4803. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论