Proteomic analysis of grape berry skin responding to sunlight exclusion

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	Proteomic analysis of grape berry skin responding to sunlight exclusion
	Niu, Ning; Cao, Yuegang; Duan, Wei; Wu, Benhong; Li, Shaohua
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2013
英文摘要	The most obvious effect of sunlight exclusion from grape clusters is the inhibition of anthocyanin biosynthesis in the berry skin so that no color develops. Two-dimensional gel electrophoresis coupled with mass spectrometry was used to characterize the proteins isolated from berry skins that developed under sunlight exclusion versus those from sunlight-exposed berries. Among more than 1500 spots resolved in stained gels, the accumulation patterns of 96 spots differed significantly between sunlight-excluded berry skin and that of sunlight-exposed control berries. Seventy-two proteins, including 35 down-regulated and 37 up-regulated proteins, were identified and categorized. Proteins involved in photosynthesis and secondary metabolism, especially UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT), the key step for anthocyanin biosynthesis in grape berry skin, were accumulated less in the absence of sunlight. Several isoforms of heat shock proteins were also down-regulated. The proteins that were over-accumulated in sunlight-excluded berry skin were more often related to energy production, glycolysis, the tricarboxylicacid cycle, protein synthesis and biogenesis of cellular components. Their putative role is discussed in terms of their relevance to sunlight exclusion processes. Crown Copyright (C) 2013 Published by Elsevier GmbH. All rights reserved.
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/615]
专题	中国科学院武汉植物园
推荐引用方式 GB/T 7714	Niu, Ning,Cao, Yuegang,Duan, Wei,et al. Proteomic analysis of grape berry skin responding to sunlight exclusion[J]. JOURNAL OF PLANT PHYSIOLOGY,2013.
APA	Niu, Ning,Cao, Yuegang,Duan, Wei,Wu, Benhong,&Li, Shaohua.(2013).Proteomic analysis of grape berry skin responding to sunlight exclusion.JOURNAL OF PLANT PHYSIOLOGY.
MLA	Niu, Ning,et al."Proteomic analysis of grape berry skin responding to sunlight exclusion".JOURNAL OF PLANT PHYSIOLOGY (2013).