Flower Colour Modification of Chrysanthemum by Suppression of F3 ' H and Overexpression of the Exogenous Senecio cruentus F3 ' 5 ' H Gene

CORC > 武汉植物园 > 中国科学院武汉植物园

	Flower Colour Modification of Chrysanthemum by Suppression of F3 ' H and Overexpression of the Exogenous Senecio cruentus F3 ' 5 ' H Gene
	Huang He; Hu Ke; Dai Silan; Xiang Qiaoyan; Han Keting
刊名	PLOS ONE
	2013
英文摘要	Chrysanthemum (Chrysanthemum x morifolium) is one of the most important ornamental plants in the world. They are typically used as cut flowers or potted plants. Chrysanthemum can exhibit red, purple, pink, yellow and white flowers, but lack bright red and blue flowers. In this study, we identified two chrysanthemum cultivars, C x morifolium 'LPi' and C x morifolium 'LPu', that only accumulate flavonoids in their ligulate flowers. Next, we isolated seven anthocyanin biosynthesis genes, namely CmCHS, CmF3H, CmF3'H, CmDFR, CmANS, CmCHI and Cm3GT in these cultivars. RT-PCR and qRT-PCR analyses showed that CmF3'H was the most important enzyme required for cyanidin biosynthsis. To rebuild the delphinidin pathway, we downregulated CmF3'H using RNAi and overexpressed the Senecio cruentus F3'5'H (PCFH) gene in chrysanthemum. The resultant chrysanthemum demonstrated a significantly increased content of cyanidin and brighter red flower petals but did not accumulate delphinidin. These results indicated that CmF3'H in chrysanthemum is important for anthocyanin accumulation, and Senecio cruentus F3'5'H only exhibited F3' H activity in chrysanthemum but did not rebuild the delphinidin pathway to form blue flower chrysanthemum.
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/536]
专题	中国科学院武汉植物园
推荐引用方式 GB/T 7714	Huang He,Hu Ke,Dai Silan,et al. Flower Colour Modification of Chrysanthemum by Suppression of F3 ' H and Overexpression of the Exogenous Senecio cruentus F3 ' 5 ' H Gene[J]. PLOS ONE,2013.
APA	Huang He,Hu Ke,Dai Silan,Xiang Qiaoyan,&Han Keting.(2013).Flower Colour Modification of Chrysanthemum by Suppression of F3 ' H and Overexpression of the Exogenous Senecio cruentus F3 ' 5 ' H Gene.PLOS ONE.
MLA	Huang He,et al."Flower Colour Modification of Chrysanthemum by Suppression of F3 ' H and Overexpression of the Exogenous Senecio cruentus F3 ' 5 ' H Gene".PLOS ONE (2013).