Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis

doi:10.1016/j.jprot.2015.11.004

CORC > 武汉植物园 > 中国科学院武汉植物园

	Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis
	Li, Ming 1; Wang, Kun 2; Li, Shaoqing 2; Yang, Pingfang 1
刊名	JOURNAL OF PROTEOMICS
	2016-01-10
卷号	131 页码:214-226
关键词	Oryza sativa L. Pistil Pollination Reactive oxygen species Proteomic Transcriptomic
ISSN号	1874-3919
DOI	10.1016/j.jprot.2015.11.004
英文摘要	Pollen-stigma interaction is a multi-step and complex physiological process which contains different signaling and biochemical pathways. However, little is known about the molecular mechanism underlying this process in rice (Oryza saliva). In this study, the changes of gene expression were investigated through a combination study of transcriptome and proteome profiles in rice pistil during pollination. Totally, 1117 differentially expressed genes were identified, among which 962 and 167 were detected at transcriptional and protein level respectively. Functional categorization analysis showed that the genes involved in central metabolism were up-regulated, which can lead to the enhancement of these metabolisms. The reactive oxygen species (ROS) were over-accumulated in the stigma. In response to this, the proteins or transcripts involved in redox homeostasis regulation were differentially expressed. Furthermore, significant changes of protein ubiquitination and its related genes or proteins, especially some E3 ligases encoding genes, indicate that protein ubiquitination might play important roles in cell signal transduction during the pollination process. Our study sheds some lights on gene and protein expression profiles of rice pistil pollination process, and gives us a comprehensive understanding of the basic molecular mechanisms controlling pollination in rice. Biological significance: Using RNA-seq, 2-DE and iTRAQ assays, we have generated the large-scale transcriptomic and proteomic data containing abundant information on genes involved in pollen and pistil interaction. Our results showed that ROS were significantly accumulated in stigma after pollination, and the abundance of genes involve in redox homeostasis system were changed variously. We also show that, changes of some E3 ligases encoding genes might indicate that protein ubiquitination play important roles in cell signal transduction during the pollination process. Data in this study might be helpful to deeply understand the pollination in rice. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[31100230] ; 100 talents project from Chinese Academy of Sciences ; Open Research Fund of State Key Laboratory of Hybrid Rice, Wuhan University[KF201306]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000366540200022
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/137]
专题	中国科学院武汉植物园
通讯作者	Li, Shaoqing
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li, Ming,Wang, Kun,Li, Shaoqing,et al. Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis[J]. JOURNAL OF PROTEOMICS,2016,131:214-226.
APA	Li, Ming,Wang, Kun,Li, Shaoqing,&Yang, Pingfang.(2016).Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis.JOURNAL OF PROTEOMICS,131,214-226.
MLA	Li, Ming,et al."Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis".JOURNAL OF PROTEOMICS 131(2016):214-226.