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Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit
Liu, Chunyan1,2; Huang, Hongwen2; Yan, Chunlin1,2; Zhong, Caihong2; Yao, Xiaohong2; Zhang, Qiong2
刊名TREE GENETICS & GENOMES
2016-04-01
卷号12期号:2页码:9
关键词Actinidia SSRs Linkagemap Sex-linked markers Interspecific hybridization
ISSN号1614-2942
DOI10.1007/s11295-016-0982-2
英文摘要Simple sequence repeats (SSRs) have been widely used in the construction of linkage maps, quantitative trait loci (QTLs) mapping, and marker-assisted selection (MAS). The availability of the sequenced Actinidia chinensis (kiwifruit) genome allows for the inexpensive and efficient development of microsatellite markers. In this study, a total of 49,067 SSRs were identified and characterized in the genome sequences of kiwifruit. Dinucleotide repeats are the most abundant SSRs, with the AG/TC motif accounting for 44.2 % of all SSRs in the genome. Fifty-five newly derived SSRs, together with 46 previously available SSRs, were integrated into linkage maps of an interspecific kiwifruit population. In addition, eight sex-linked SSR markers (including one previously published SSR) were mapped in the sex-related region on the LG25, suggesting that recombination is partially suppressed to maintain dioecy in kiwifruit. The SSRs developed from this study are a valuable resource for kiwifruit genetics and will contribute to the use of MAS in early sex determination of dioecious plant breeding.
资助项目National Natural Science Foundation of China[31301749] ; Wuhan Applied Basic Research Project[2015020101010075]
WOS研究方向Forestry ; Genetics & Heredity ; Agriculture
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000372272300007
内容类型期刊论文
源URL[http://202.127.146.157/handle/2RYDP1HH/98]  
专题中国科学院武汉植物园
通讯作者Huang, Hongwen
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Liu, Chunyan,Huang, Hongwen,Yan, Chunlin,et al. Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit[J]. TREE GENETICS & GENOMES,2016,12(2):9.
APA Liu, Chunyan,Huang, Hongwen,Yan, Chunlin,Zhong, Caihong,Yao, Xiaohong,&Zhang, Qiong.(2016).Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit.TREE GENETICS & GENOMES,12(2),9.
MLA Liu, Chunyan,et al."Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit".TREE GENETICS & GENOMES 12.2(2016):9.
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