Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit | |
Liu, Chunyan1,2; Huang, Hongwen2; Yan, Chunlin1,2; Zhong, Caihong2; Yao, Xiaohong2; Zhang, Qiong2 | |
刊名 | TREE GENETICS & GENOMES |
2016-04-01 | |
卷号 | 12期号:2页码:9 |
关键词 | Actinidia SSRs Linkagemap Sex-linked markers Interspecific hybridization |
ISSN号 | 1614-2942 |
DOI | 10.1007/s11295-016-0982-2 |
英文摘要 | Simple sequence repeats (SSRs) have been widely used in the construction of linkage maps, quantitative trait loci (QTLs) mapping, and marker-assisted selection (MAS). The availability of the sequenced Actinidia chinensis (kiwifruit) genome allows for the inexpensive and efficient development of microsatellite markers. In this study, a total of 49,067 SSRs were identified and characterized in the genome sequences of kiwifruit. Dinucleotide repeats are the most abundant SSRs, with the AG/TC motif accounting for 44.2 % of all SSRs in the genome. Fifty-five newly derived SSRs, together with 46 previously available SSRs, were integrated into linkage maps of an interspecific kiwifruit population. In addition, eight sex-linked SSR markers (including one previously published SSR) were mapped in the sex-related region on the LG25, suggesting that recombination is partially suppressed to maintain dioecy in kiwifruit. The SSRs developed from this study are a valuable resource for kiwifruit genetics and will contribute to the use of MAS in early sex determination of dioecious plant breeding. |
资助项目 | National Natural Science Foundation of China[31301749] ; Wuhan Applied Basic Research Project[2015020101010075] |
WOS研究方向 | Forestry ; Genetics & Heredity ; Agriculture |
语种 | 英语 |
出版者 | SPRINGER HEIDELBERG |
WOS记录号 | WOS:000372272300007 |
内容类型 | 期刊论文 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/98] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Huang, Hongwen |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Chunyan,Huang, Hongwen,Yan, Chunlin,et al. Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit[J]. TREE GENETICS & GENOMES,2016,12(2):9. |
APA | Liu, Chunyan,Huang, Hongwen,Yan, Chunlin,Zhong, Caihong,Yao, Xiaohong,&Zhang, Qiong.(2016).Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit.TREE GENETICS & GENOMES,12(2),9. |
MLA | Liu, Chunyan,et al."Characterization of genome-wide simple sequence repeats and application in interspecific genetic map integration in kiwifruit".TREE GENETICS & GENOMES 12.2(2016):9. |
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