Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera)

doi:10.3389/fpls.2016.00750

CORC > 武汉植物园 > 中国科学院武汉植物园

	Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera)
	Wang, Lei 4; Fu, Jinlei 1; Li, Ming 1; Weckwerth, Wolfram 3,4; Yang, Pingfang 1,2; Fragner, Lena 4
刊名	FRONTIERS IN PLANT SCIENCE
	2016-06-07
卷号	7 页码:12
关键词	mass spectrometry primary metabolism GC-TOF-MS LC-Orbitrap-MS seed development Nelumbo nucifera
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00750
英文摘要	Sacred lotus (Nelumbo nucifera) belongs to the Nelumbonaceae family. Its seeds are widely consumed in Asian countries as snacks or even medicine. Besides the market value, lotus seed also plays a crucial role in the lotus life cycle. Consequently, it is essential to gain a comprehensive understanding of the development of lotus seed. During its development, lotus seed undergoes cell division, expansion, reserve accumulation, desiccation, and maturation phases. We observed morphological and biochemical changes from 10 to 25 days after pollination (DAP) which corresponded to the reserve synthesis and accumulation phase. The volume of the seed expanded until 20 DAP with the color of the seed coat changing from yellow-green to dark green and gradually fading again. Starch and protein rapidly accumulated from 15 to 20 DAP. To further reveal metabolic adaptation, primary metabolites and proteins profiles were obtained using mass spectrometry based platforms. Metabolites and enzymes involved in sugar metabolism, glycolysis, TCA cycle and amino acid metabolism showed sequential dynamics enabling the clear separation of the different metabolic states during lotus seed development. The integration of the data revealed a highly significant metabolic switch at 15 DAP going through a transition of metabolically highly active tissue to the preparation of storage tissue. The results provide a reference data set for the evaluation of primary metabolism during lotus seed development.
资助项目	Chinese Academy of Sciences[Y455421Z02] ; China Scholarship Council (CSC)[201206220134]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000377257700001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/62]
专题	中国科学院武汉植物园
通讯作者	Weckwerth, Wolfram; Yang, Pingfang
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan, Peoples R China 2.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan, Peoples R China 3.Univ Vienna, Vienna Metab Ctr, Vienna, Austria 4.Univ Vienna, Dept Ecogen & Syst Biol, Vienna, Austria
推荐引用方式 GB/T 7714	Wang, Lei,Fu, Jinlei,Li, Ming,et al. Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera)[J]. FRONTIERS IN PLANT SCIENCE,2016,7:12.
APA	Wang, Lei,Fu, Jinlei,Li, Ming,Weckwerth, Wolfram,Yang, Pingfang,&Fragner, Lena.(2016).Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera).FRONTIERS IN PLANT SCIENCE,7,12.
MLA	Wang, Lei,et al."Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera)".FRONTIERS IN PLANT SCIENCE 7(2016):12.