Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02

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	Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02
	GYIG-IR管理员
刊名	African Journal of Biotechnology》 , ,
	2011
卷号	10 期号:42 页码:8403-8413
关键词	Bacillus Mucilaginosus Carbonic Anhydrase Specific Production Rate Kinetic Analysis Orthogonal
英文摘要	The objective of this study was to optimize the submerged cultured conditions of carbonic anhydrase (CA) production from the strain of Bacillus mucilaginosus K02 by mono-factor tests with blank control and orthogonal design methodology. When the bacteria was cultured under the condition of monofactor tests with blank control and orthogonal designs, and conventional mono-factor test and orthogonal designs, respectively, the CA productions increased from 5.32 µU/L under the basic medium to 34.53 and 26.12 µU/L. The kinetic analysis of 5 L-fermentor showed that the specific production rate of CA in the optimized medium was almost equaled to the one obtained in the basic and optimized medium with conventional methods. The result demonstrated that CA synthesis was positively correlated with cell growth, and the mono-factor tests with blank control applied in the optimizing composition of the media could achieve better results in contrast to the conventional method.
学科主题	环境地球化学
语种	英语
公开日期	2017-12-27
内容类型	期刊论文
源URL	[http://ir.gyig.ac.cn:8080/handle/352002/6970]
专题	地球化学研究所_环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
推荐引用方式 GB/T 7714	GYIG-IR管理员. Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02[J]. African Journal of Biotechnology》 , ,,2011,10(42):8403-8413.
APA	GYIG-IR管理员.(2011).Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02.African Journal of Biotechnology》 , ,,10(42),8403-8413.
MLA	GYIG-IR管理员."Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02".African Journal of Biotechnology》 , , 10.42(2011):8403-8413.