Determination of total iron binding capacity of serum by capillary electrophoresis

	Determination of total iron binding capacity of serum by capillary electrophoresis
	Ding, YS ; Liu, LL ; Ma, YF ; Lin, BC
刊名	chromatographia
	1999
卷号	49 期号:1-2 页码:71-74
关键词	capillary electrophoresis serum analysis total iron binding capacity
英文摘要	a capillary electrophoresis (ce) technique for determining total iron binding capacity (tibc) of serum has been developed. the optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with fe+3; then, dissociate them completely after removing excess unbound fe. finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the ce analysis. ammonium acetate (ph = 5.0) was used as ce background electrolyte solution. in this system, a good linear correlation coefficient was maintained over the range 0.5 similar to 10 mu m (r = 0.9979, n =12). seven adult serum samples were studied and the tibc parameters measured. in the present system, 10 similar to 30 mu l serum is sufficient for determination. the study shows that the ce technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	transferrin receptor levels ; rheumatoid-arthritis ; performance ; separation ; proteins ; anemia
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000078308200012
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/86171]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Truman State Univ, Div Sci, Kirksville, MO 63501 USA
推荐引用方式 GB/T 7714	Ding, YS,Liu, LL,Ma, YF,et al. Determination of total iron binding capacity of serum by capillary electrophoresis[J]. chromatographia,1999,49(1-2):71-74.
APA	Ding, YS,Liu, LL,Ma, YF,&Lin, BC.(1999).Determination of total iron binding capacity of serum by capillary electrophoresis.chromatographia,49(1-2),71-74.
MLA	Ding, YS,et al."Determination of total iron binding capacity of serum by capillary electrophoresis".chromatographia 49.1-2(1999):71-74.