Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant

	Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant
	Ye, ML; Zou, HF; Liu, Z; Ni, JY; Zhang, YK
刊名	journal of chromatography a
	1999-09-03
卷号	855 期号:1 页码:137-145
关键词	electrochromatography dynamically modified silica capillary electrochromatography stationary phases anilines benzenes peptides LC
英文摘要	a novel mode of capillary electrochromatography (cec), called dynamically modified silica-capillary electrochromatography, is described in this paper. the column packed with bare silica was dynamically modified with long chain quaternary ammonium salt, cetyltrimethylammonium bromide (ctab), which was added into the mobile phase. ctab ions were adsorbed onto the surface of bare silica, and the resulted hydrophobic layer on the silica gel was used as the stationary phase; using the dynamically modified silica column, neutral solutes were separated by cec. the highest number of theoretical plates obtained was about 71 500/m and the relative standard deviations for t(0) and capacity factor of toluene were 4.7% and 4.9% for 20 consecutive runs, respectively. the separation mechanism of neutral solutes and the influence of mobile phase composition on the separation was investigated. the separation of nitrogen-containing solutes was carried out with this mode and the peak tailing of basic solute was effectively eliminated because the adsorption of basic solute on silica was blocked by the preferred adsorption of ctab. (c) 1999 elsevier science b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; stationary phases ; separation ; behavior ; electrophoresis ; bases
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000082604000008
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/85601]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Ye, ML,Zou, HF,Liu, Z,et al. Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant[J]. journal of chromatography a,1999,855(1):137-145.
APA	Ye, ML,Zou, HF,Liu, Z,Ni, JY,&Zhang, YK.(1999).Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant.journal of chromatography a,855(1),137-145.
MLA	Ye, ML,et al."Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant".journal of chromatography a 855.1(1999):137-145.