LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization

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	LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
	Sun, Jing 1; Li, Faqiang 2; Xu, Wenhua 1; Zhou, Guoying 1; You, Jinmao 1,3; Chen, Guichen 1
刊名	CHROMATOGRAPHIA
	2009-12-01
卷号	70 期号:11-12 页码:1627-1633
关键词	Column Liquid Chromatography Lc-esi-ms Free Amino Acids Tibetan Medicine Gentiana Dahurica
文献子类	Article
英文摘要	Concentrations of 20 free amino aicds (FAAs) in a famous Tibetan medicine Gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (RP-LC). 20 amino acid derivatives (AAD) were separated on a Hypersil BDS C(18) column with a good baseline resolution within 65 min. Identification of 20 AAD was by online post-column mass spectrometry with an electrospray ionization (ESI) source. The validation of the method was examined by linearity, repeatability, and detection limits. Most linear correlation coefficients for AAD were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. There were 18 FAAs found in G. dahurica, of which seven FAAs were necessary to the people's health and related to the treatment of liver and gall disease. Variation of concentrations of the 20 FAAs showed geographical distribution difference among populations. Meanwhile a stable genetic diversity of FAAs composition of G. dahurica was also revealed at the species level. Results of the present study proved that the established method was rapid and reproducible for further separation and determination of FAAs in more medicinal plants.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; CHLOROFORMATE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000272173800016
内容类型	期刊论文
源URL	[http://ir.isl.ac.cn/handle/363002/5908]
专题	青海盐湖研究所_青海盐湖研究所知识仓储青海盐湖研究所_盐湖资源与化学实验室
作者单位	1.Chinese Acad Sci, Res Ctr Natl Tibetan Med, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jing,Li, Faqiang,Xu, Wenhua,et al. LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization[J]. CHROMATOGRAPHIA,2009,70(11-12):1627-1633.
APA	Sun, Jing,Li, Faqiang,Xu, Wenhua,Zhou, Guoying,You, Jinmao,&Chen, Guichen.(2009).LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization.CHROMATOGRAPHIA,70(11-12),1627-1633.
MLA	Sun, Jing,et al."LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization".CHROMATOGRAPHIA 70.11-12(2009):1627-1633.