Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1

doi:10.1007/s10811-017-1308-y

	Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1
	Yuan, Cheng 2; Xu, Kai 1,2; Sun, Juan 1; Hu, Guang-Rong 2; Li, Fu-2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2018-04-01
卷号	30 期号:2 页码:793-801
关键词	Chlorophyta Nitrogen Sources Lipid Ph Adjustment Nervonic Acid Mychonastes Afer
ISSN号	0921-8971
DOI	10.1007/s10811-017-1308-y
文献子类	Article
英文摘要	Producing valuable coproducts from oleaginous microalgae is an option to reduce the total cost of biofuel production. Here, the influence of nitrogen sources on biomass yield and lipid accumulation of a newly identified oleaginous green microalgal strain, Mychonastes afer HSO-3-1, was evaluated. Carbon assimilation and the following lipid biosynthesis of M. afer were inhibited to some extent under weak acidic conditions (6 < pH < 7) and any of the tested nitrogen source. The highest lipid productivity of 50.7 mg L-1 day(-1) was achieved with a 17.6 mM nitrogen supplement in the form of urea. The cell polar lipid content was significantly higher than triacylglycerol (TAG), and saturated palmitic acid (C16:0) occupied a dominant position in the fatty acid profiles while culturing M. afer in acidic medium with NH4 (+) as the nitrogen source. Under neutral conditions, the lipid productivities of M. afer cultivated in media containing 17.6 mM of NaNO3, NH4Cl, and NH4NO3 were 76.2, 77.5, and 79.0 mg L-1 day(-1), respectively. The greatest TAG content (58.56%) of total lipids was obtained when NaNO3 was used as the nitrogen source. There was no significant difference in the fatty acid composition of M. afer cells when they were cultivated in neutral media supplemented with NaNO3, urea, NH4Cl, and NH4NO3. Therefore, NH4 (+) was not a suitable nitrogen source for M. afer cultivation due to the additional labor, working procedures, and alkali required to adjust the medium pH. Considering that using urea as nitrogen source could reduce the cost of nutrient salts substantially and urea can be taken up and utilized by most microalgae, it is a preferred nitrogen source. The major properties of biodiesel derived from M. afer HSO-3-1 met biodiesel quality, and nervonic acid concentrations remained at approximately 3.0% of total fatty acids.
WOS关键词	ARTHROSPIRA SPIRULINA PLATENSIS ; DIATOM NITZSCHIA-LAEVIS ; FED-BATCH CULTIVATION ; NITROGEN-SOURCE ; BIODIESEL PRODUCTION ; TUBULAR PHOTOBIOREACTOR ; CHLORELLA-VULGARIS ; HETEROTROPHIC PRODUCTION ; ALCOHOLIC FERMENTATION ; EICOSAPENTAENOIC ACID
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000431194900005
资助机构	Ministry of Science and Technology of China(2014AA022003) ; earmarked fund for China Agriculture Research System(CARS-35-33) ; National key research and development program-China(2016YFB0601001) ; Key R&D Program of Shandong Province(2015GSF115027)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11513]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Juan
作者单位	1.Qingdao Agr Univ, Inst Econ Herb Plants, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Cheng,Xu, Kai,Sun, Juan,et al. Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(2):793-801.
APA	Yuan, Cheng,Xu, Kai,Sun, Juan,Hu, Guang-Rong,&Li, Fu-.(2018).Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1.JOURNAL OF APPLIED PHYCOLOGY,30(2),793-801.
MLA	Yuan, Cheng,et al."Ammonium, nitrate, and urea play different roles for lipid accumulation in the nervonic acid-producing microalgae Mychonastes afer HSO-3-1".JOURNAL OF APPLIED PHYCOLOGY 30.2(2018):793-801.