Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis

doi:10.1186/s12934-018-0913-z

	Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis
	Chen, Hailin 1,2; Li, Meijie 1; Liu, Changqing 1; Zhang, Haibo 1; Xian, Mo 1; Liu, Huizhou 1
刊名	MICROBIAL CELL FACTORIES
	2018-04-30
卷号	17
关键词	Isopentenyl Diphosphate Isomerase Random Mutagenesis Enzyme Activity Lycopene Production Mevalonate Pathway
ISSN号	1475-2859
DOI	10.1186/s12934-018-0913-z
文献子类	Article
英文摘要	Background: Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. 5.3.3.2) is one of the rate-limiting enzymes in the lycopene biosynthetic pathway and one major target during metabolic engineering. The properties of IDIs differ depending on the sources, but under physiological conditions, IDIs are limited by low enzyme activity, short half-life and weak substrate affinity. Therefore, it is important to prepare an excellent IDI by protein engineering.
WOS关键词	MEVALONATE-INDEPENDENT PATHWAY ; DIMETHYLALLYL DIPHOSPHATE ; ESCHERICHIA-COLI ; STREPTOCOCCUS-PNEUMONIAE ; ISOPRENOID BIOSYNTHESIS ; CRYSTAL-STRUCTURE ; LYCOPENE ; EVOLUTION ; CHLOROPLASTS ; INHIBITION
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000431339000002
资助机构	National Natural Science Foundation of China(21572242) ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; National Natural Science Foundation of China (NSF)(31400084) ; Youth Innovation Promotion Association CAS(2017252)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11483]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhang, Haibo; Xian, Mo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Coll, 19 A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hailin,Li, Meijie,Liu, Changqing,et al. Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis[J]. MICROBIAL CELL FACTORIES,2018,17.
APA	Chen, Hailin,Li, Meijie,Liu, Changqing,Zhang, Haibo,Xian, Mo,&Liu, Huizhou.(2018).Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis.MICROBIAL CELL FACTORIES,17.
MLA	Chen, Hailin,et al."Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis".MICROBIAL CELL FACTORIES 17(2018).