Beclin-1 is involved in tongue sole Cynoglossus semilaevis immune defense against bacterial infection

doi:10.1016/j.fsi.2018.03.023

	Beclin-1 is involved in tongue sole Cynoglossus semilaevis immune defense against bacterial infection
	Li, Xue-peng 1,2; Chi, Heng 1,2; Zhang, Jian 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-06-01
卷号	77 页码:8-12
关键词	Beclin-1 Cynoglossus Semilaevis Gene Expression Immune Defense
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.03.023
文献子类	Article
英文摘要	In mammals, beclin-1 is a key player that regulates autophagic activity. In fish, the immune function of beclin-1 is essentially unknown. In this study, we analyzed the involvement of tongue sole (Cynoglossus semilaevis) beclin-1 (named CsBECN1) in antibacterial immunity. CsBECN1 is composed of 451 amino acid residues and shares 84.5-95.1% overall sequence identities with other teleost beclin-1. CsBECN1 possesses a typical Bcl-2 homology domain 3 and an Atg6 domain. Expression of CsBECN1 occurred in multiple tissues and was upregulated during bacterial infection. Knockdown of CsBECN1 significantly enhanced bacterial dissemination in the tissues of tongue sole, whereas overexpression of CsBECN1 significantly reduced bacterial dissemination. Taken together, these results indicate that CsBECN1 is required for the antibacterial immunity of tongue sole.
WOS关键词	MOLECULAR CHARACTERIZATION ; ADAPTIVE IMMUNITY ; AUTOPHAGY GENE ; UP-REGULATION ; PROTEIN ; EXPRESSION ; DEGRADATION ; INNATE ; TUMORIGENESIS ; MECHANISMS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000432644300002
资助机构	National Natural Science Foundation of China(31330081 ; Key Laboratory of Mariculture (KLM), Ministry of Education, Ocean University of China ; Taishan Scholar Program of Shandong Province ; 31502197)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11439]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhang, Jian
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Xue-peng,Chi, Heng,Zhang, Jian. Beclin-1 is involved in tongue sole Cynoglossus semilaevis immune defense against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2018,77:8-12.
APA	Li, Xue-peng,Chi, Heng,&Zhang, Jian.(2018).Beclin-1 is involved in tongue sole Cynoglossus semilaevis immune defense against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,77,8-12.
MLA	Li, Xue-peng,et al."Beclin-1 is involved in tongue sole Cynoglossus semilaevis immune defense against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 77(2018):8-12.