Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection

doi:10.3389/fmicb.2018.01084

	Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection
	Li, Mo-fei 1,2; Sun, Li 1,2
刊名	FRONTIERS IN MICROBIOLOGY
	2018-05-25
卷号	9
关键词	Edwardsiella Tarda Hydrogenase Serum Resistance Acid Resistance Virulence
ISSN号	1664-302X
DOI	10.3389/fmicb.2018.01084
文献子类	Article
英文摘要	Edwardsiella tarda is a broad-host pathogen that can infect mammals, reptiles, and fish. E. tarda exhibits a remarkable ability to survive in host serum and replicate in host phagocytes, but the underlining mechanism is unclear. In this study, in order to identify E. tarda proteins involved in serum resistance, iTRAQ proteomic analysis was performed to examine the whole-cell protein profiles of TX01, a pathogenic E. tarda isolate, in response to serum treatment. Of the differentially expressed proteins identified, one (named Sip2) possesses a conserved hydrogenase domain and is homologous to the putative hydrogenase accessory protein HypB. When Sip2 was expressed in Escherichia coli, it significantly enhanced the survival of the host cells in serum. Compared to TX01, the sip2 knockout, TX01 Delta sip2, was dramatically reduced in the ability of hydrogenase activity, serum resistance, intracellular replication, dissemination in fish tissues, and causing mortality in infected fish. The lost virulence capacities of TX01 Delta sip2 were restored by complementation with the sip2 gene. Furthermore, TX01 Delta sip2 was significantly reduced in the capacity to grow under low pHs and iron-depleted conditions, and was unable to maintain its internal pH in acidic environment. Taken together, these results indicate that Sip2 is a novel serum-induced protein that is essential to serum resistance, cellular and tissue infection, and coping with acidic stress via its ability to modulate intracellular pH.
WOS关键词	ENTERICA SEROVAR TYPHIMURIUM ; ESCHERICHIA-COLI ; COMPLEMENT-SYSTEM ; DIFFERENTIAL EXPRESSION ; HYDROGEN-PRODUCTION ; IN-VIVO ; VIRULENCE ; FORMATE ; STRAIN ; PH
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000433133300001
资助机构	National Natural Science Foundation of China(31602197 ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2017249) ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province ; 31330081)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11413]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Mo-fei,Sun, Li. Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection[J]. FRONTIERS IN MICROBIOLOGY,2018,9.
APA	Li, Mo-fei,&Sun, Li.(2018).Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection.FRONTIERS IN MICROBIOLOGY,9.
MLA	Li, Mo-fei,et al."Edwardsiella tarda Sip2: A Serum-Induced Protein That Is Essential to Serum Survival, Acid Resistance, Intracellular Replication, and Host Infection".FRONTIERS IN MICROBIOLOGY 9(2018).