Deciphering the late steps of rifamycin biosynthesis | |
Qi, Feifei1; Lei, Chao2; Li, Fengwei1; Zhang, Xingwang1; Wang, Jin2; Zhang, Wei1; Fan, Zhen2; Li, Weichao2; Tang, Gong-Li3; Xiao, Youli2,4 | |
刊名 | NATURE COMMUNICATIONS
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2018-06-14 | |
卷号 | 9 |
ISSN号 | 2041-1723 |
DOI | 10.1038/s41467-018-04772-x |
文献子类 | Article |
英文摘要 | Rifamycin-derived drugs, including rifampin, rifabutin, rifapentine, and rifaximin, have long been used as first-line therapies for the treatment of tuberculosis and other deadly infections. However, the late steps leading to the biosynthesis of the industrially important rifamycin SV and B remain largely unknown. Here, we characterize a network of reactions underlying the biosynthesis of rifamycin SV, S, L, O, and B. The two-subunit transketolase Rif15 and the cytochrome P450 enzyme Rif16 are found to mediate, respectively, a unique C-O bond formation in rifamycin L and an atypical P450 ester-to-ether transformation from rifamycin L to B. Both reactions showcase interesting chemistries for these two widespread and well-studied enzyme families. |
WOS关键词 | AMYCOLATOPSIS-MEDITERRANEI S699 ; GENE-CLUSTER ; SP NOV. ; CYTOCHROME-P450 ; P450 ; TRANSKETOLASE ; SEQUENCE ; PROTEIN ; SV ; TRANSFORMATION |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000435341000006 |
资助机构 | Shandong Provincial Natural Science Foundation(ZR2017ZB0207) ; National Natural Science Foundation of China(81741155 ; Chinese Academy of Sciences(QYZDB-SSW-SMC042 ; Science and Technology Commission of Shanghai Municipality(15JC1400402) ; 31422002 ; XDPB0402) ; 21472204 ; 31600036 ; 21572243 ; 31430004 ; 31670058) |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/11379] ![]() |
专题 | 中国科学院青岛生物能源与过程研究所 |
通讯作者 | Xiao, Youli; Li, Shengying |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Qi, Feifei,Lei, Chao,Li, Fengwei,et al. Deciphering the late steps of rifamycin biosynthesis[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Qi, Feifei.,Lei, Chao.,Li, Fengwei.,Zhang, Xingwang.,Wang, Jin.,...&Li, Shengying.(2018).Deciphering the late steps of rifamycin biosynthesis.NATURE COMMUNICATIONS,9. |
MLA | Qi, Feifei,et al."Deciphering the late steps of rifamycin biosynthesis".NATURE COMMUNICATIONS 9(2018). |
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