Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics
	Zhou, Jin-Wei 1,2; Hou, Bo 3,4; Liu, Gen-Yan 5; Jiang, Huan 1; Sun, Bing 1; Wang, Zhen-Nan 1; Shi, Ruo-Fu 1; Xu, Yuan 6; Wang, Rong 2; Jia, Ai-Qun 2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2018-11-01
卷号	102 期号:22 页码:9745-9758
关键词	Pseudomonas aeruginosa Biofilm Quorum sensing Susceptibility Antibiotics
ISSN号	0175-7598
英文摘要	Pseudomonas aeruginosa is a ubiquitous pathogen that is the leading cause of chronic infections. Bacterial biofilm formation facilitates CF development and restricts the anti-bacterial potential of many current antibiotics. The capacity of P. aeruginosa to form biofilms and resist antibiotics is closely correlated with quorum sensing (QS). Disrupting QS by QS inhibitors is a promising strategy for treating chronic infections. Here, we evaluated the effect of hordenine, a recently characterized QS inhibitor, on the susceptibility of aminoglycoside antibiotics against P. aeruginosa biofilms. Hordenine significantly enhanced the susceptibility of aminoglycoside antibiotics tobramycin, gentamycin, and amikacin against P. aeruginosa PAO1 biofilm formation. Combinations of hordenine and aminoglycoside antibiotics showed potent efficiency in disrupting the preformed biofilms of P. aeruginosa. Microscopic observations showed flat, scattered, and unstructured biofilm architecture after treatment with hordenine. Mechanistic study further revealed that hordenine treatment led to the downregulation of genes involved in QS and biofilm formation. Thus, our results suggest that hordenine has the potential to function as an antibiotic accelerant in treating P. aeruginosa infections.
资助项目	National Key Research and Development Program of China[2017YFD0201401] ; National Natural Science Foundation of China[41766006] ; Natural Science Foundation of Jiangsu Province, China[BK20170859] ; Science and Technology Development Program of Modern Agriculture, Nanjing[201608052] ; Six Talent Peaks Project in Jiangsu Province ; Fundamental Research Funds for the Central Universities[30916011307]
语种	英语
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province, China ; Science and Technology Development Program of Modern Agriculture, Nanjing ; Six Talent Peaks Project in Jiangsu Province ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/63094]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Wang, Rong; Jia, Ai-Qun
作者单位	1.Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China 2.Hainan Univ, Key Lab Trop Biol Resources, Minist Educ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Yunnan Key Lab Nat Med Chem, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 5.Nanjing Med Univ, Dept Lab Med, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China 6.Nanjing Univ Chinese Med, Sch Med & Life Sci, 138 Xianlin Rd, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jin-Wei,Hou, Bo,Liu, Gen-Yan,et al. Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(22):9745-9758.
APA	Zhou, Jin-Wei.,Hou, Bo.,Liu, Gen-Yan.,Jiang, Huan.,Sun, Bing.,...&Jia, Ai-Qun.(2018).Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(22),9745-9758.
MLA	Zhou, Jin-Wei,et al."Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.22(2018):9745-9758.