A Supersensitive CTC Analysis System Based on Triangular Silver Nanoprisms and SPION with Function of Capture, Enrichment, Detection, and Release

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2018专题

	A Supersensitive CTC Analysis System Based on Triangular Silver Nanoprisms and SPION with Function of Capture, Enrichment, Detection, and Release
	Ruan, Huimin; Wu, Xiaoxia; Yang, Chengcheng; Li, Zihou; Xia, Yuanzhi; Xue, Ting; Shen, Zheyu; Wu, Aiguo
刊名	ACS BIOMATERIALS SCIENCE & ENGINEERING
	2018
卷号	4 期号:3 页码:1073-1082
关键词	Circulating Tumor-cells Cancer-cells Colorimetric Detection Efficient Capture High Specificity Nanoparticles Sers Technologies Nanospheres Recognition
英文摘要	Detection of circulating tumor cells (CTCs) may be applied for diagnosis of early tumors like a liquid biopsy. However, the sensitivity remains a challenge because CTCs are extremely rare in peripheral blood. In this study, we developed a supersensitive CTC analysis system based on triangular silver nanoprisms (AgNPR) and superparamagnetic iron oxide nanoparticles (SPION) with function of capture, enrichment, detection, and release. The AgNPR was encoded with MBA (i.e., 4-mercaptobenzoic acid) and modified with rBSA (i.e., reductive bovine serum albumin) and FA (i.e., folic acid) generating organic/inorganic composite nanoparticle MBA-AgNPR-rBSA-FA, which has the function of surface-enhanced Raman scattering (SERS). The optimized SERS nanoparticles (i.e., MBA3-AgNPR-rBSA4-FA2) can be utilized for CTC detection in blood samples with high sensitivity and specificity, and the LOD (i.e., limit of detection) reaches to five cells per milliliter. In addition, the SPION was also modified with rBSA and FA generating magnetic nanoparticle SPION-rBSA-FA. Our supersensitive CTC analysis system is composed of MBA3-AgNPR-rBSA4-FA2 and SPION-rBSA-FA nanoparticles, which were applied for capture (via interaction between FA and FR alpha), enrichment (via magnet), and detection (via SERS) of cancer cells from blood samples. The results demonstrate that our supersensitive CTC analysis system has a better sensitivity and specificity than the SERS nanoparticles alone, and the LOD is up to 1 cell/mL. The flow cytometry and LSCM (i.e., laser scanning confocal microscope) results indicate the CTCs captured, enriched, and isolated by our supersensitive CTC analysis system can also be further released (via adding excessive free FA) for further cell expansion and phenotype identification.
学科主题	Materials Science ; Physics
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/16775]
专题	2018专题
推荐引用方式 GB/T 7714	Ruan, Huimin,Wu, Xiaoxia,Yang, Chengcheng,et al. A Supersensitive CTC Analysis System Based on Triangular Silver Nanoprisms and SPION with Function of Capture, Enrichment, Detection, and Release[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2018,4(3):1073-1082.
APA	Ruan, Huimin.,Wu, Xiaoxia.,Yang, Chengcheng.,Li, Zihou.,Xia, Yuanzhi.,...&Wu, Aiguo.(2018).A Supersensitive CTC Analysis System Based on Triangular Silver Nanoprisms and SPION with Function of Capture, Enrichment, Detection, and Release.ACS BIOMATERIALS SCIENCE & ENGINEERING,4(3),1073-1082.
MLA	Ruan, Huimin,et al."A Supersensitive CTC Analysis System Based on Triangular Silver Nanoprisms and SPION with Function of Capture, Enrichment, Detection, and Release".ACS BIOMATERIALS SCIENCE & ENGINEERING 4.3(2018):1073-1082.