Ramie is a typical low-cost and sustainable natural biomass resource in China. In this study, we fabricated nitrogen-doped activated carbon with high surface area from ramie biomass as a highly efficient electrocatalyst for hydrogen peroxide production. Electrochemical characterizations revealed that the as-prepared catalyst showed superior oxygen reduction reaction activity through a two-electron pathway and extraordinary long-term stability. The current density and onset potential of AC700 was greater than that of other as-prepared samples. The nitrogen atoms on the catalyst surface was derived from the biomass and deconvoluted by XPS techniques, which is beneficial for the enhancement of electrocatalytic performance. The present work furthers the development of biomass-derived carbon material for environmental applications. (C) 2018 The Electrochemical Society.