CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis
Samak, Nadia A.1,2,4; Tan, Yeqiang3; Sui, Kunyan3; Xia, Ting-Ting1,2,4; Wang, Kefeng1,2,4; Guo, Chen1,2; Liu, Chunzhao1,2,3
刊名MOLECULAR CATALYSIS
2018-02-01
卷号445页码:269-278
关键词Immobilization Magnetic Graphene Oxide Mgo-nta-cu2++ Mgo-nta-ni2++ Congo Red
ISSN号2468-8231
DOI10.1016/j.mcat.2017.12.004
文献子类Article
英文摘要In this work, a novel type of functionalized magnetic graphene oxide (MGO) nano-sheets which have the ability to capture His-tagged CotA laccase was synthesized. N alpha,N alpha-Bis(carboxymethy1)-L-lysine hydrate (NTA-NH2) was attached on MGO then chelated with Ni2+ and Cu2+ separately. The Cu2+-chelated MGO (MGO-NTA-Cu2+) exhibited the highest adsorption capacity of 177 mg/g-support when compared with both MGO and Ni2+-chelated MGO (MGO-NTA-Ni2+) which exhibited an adsorption capacity of 49.9 and 145 mg/g-support, respectively. CotA laccase immobilized on MGO-NTA-Cu2+ nano-sheets showed the highest activity recovery when compared with that immobilized on MGO-NTA-Ni2+ nano-sheets. The catalytic properties of MGO-NTA-Cu-CotA laccase were significantly improved in comparison with those of the free laccase. MGO-NTA-Cu-CotA laccase showed efficient decolorization for Congo Red (CR), and the decolorization rate reached 100% after 5 h reaction at 60 degrees C and pH 8. Furthermore, MGO-NTA-Cu-CotA laccase retained 89.4% of its initial activity after 10 consecutive cycles. Together these results indicated that the Cu2+ chelated MGO nano-sheets are promising support for efficient CotA laccase immobilization for environmental and industrial applications under high temperature and alkaline condition. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词CHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR LACCASE ; AQUEOUS-SOLUTION ; ENZYME-ACTIVITY ; DYE ; NANOPARTICLES ; REMOVAL ; ADSORPTION ; STABILITY ; ACID
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000424724500031
资助机构National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government
内容类型期刊论文
源URL[http://ir.ipe.ac.cn/handle/122111/24064]  
专题过程工程研究所_生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Qingdao Univ, Sch Mat Sci & Engn, Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Samak, Nadia A.,Tan, Yeqiang,Sui, Kunyan,et al. CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis[J]. MOLECULAR CATALYSIS,2018,445:269-278.
APA Samak, Nadia A..,Tan, Yeqiang.,Sui, Kunyan.,Xia, Ting-Ting.,Wang, Kefeng.,...&Liu, Chunzhao.(2018).CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis.MOLECULAR CATALYSIS,445,269-278.
MLA Samak, Nadia A.,et al."CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis".MOLECULAR CATALYSIS 445(2018):269-278.
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