Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production

doi:10.1007/s12649-016-9824-6

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	Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production
	Samak, Nadia A.1,2,3; Hu, Jianhua 1,2,3; Wang, Kefeng 1,2,3; Guo, Chen 1,2; Liu, Chunzhao 1,2
刊名	WASTE AND BIOMASS VALORIZATION
	2018-03-01
卷号	9 期号:3 页码:369-377
关键词	Bacillus Subtilis Bacterial Laccase Cota Iptg Micro-aerobic Condition Ros
ISSN号	1877-2641
DOI	10.1007/s12649-016-9824-6
文献子类	Article
英文摘要	Recently, bacterial laccases has drawn researchers' interest due to their ability to overcome high pH and salt concentration conditions compared to fungal laccases. Here we report a novel micro-aerobic cultivation strategy for enhancing CotA laccase expression and study its application for dye decolorization. Micro-aerobic cultivation of Escherichia coli BL21 (DE3) strain carrying pT7-FLAG-MAT-TAG-1-CotA had significantly enhanced CotA laccase activity up to 13903 U/L. The most unique findings of this investigation are that micro-aerobic cultivation strategy enhanced the reactive oxygen species production which consequently led to the over expression of CotA laccase gene. Malachite green, Crystal violet, Congo red and Bromophenol blue were efficiently decolorized by using purified CotA laccase without presence of any mediators at pH 6 and 9. These results provide a great platform for the dynamic production and application of bacterial laccase in industry.
WOS关键词	RECOMBINANT ESCHERICHIA-COLI ; GENE-EXPRESSION ; BACILLUS-LICHENIFORMIS ; OXIDATIVE STRESS ; WASTE-WATER ; DECOLORIZATION ; CLONING ; DYES ; PROTEINS ; ACCUMULATION
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000425332300004
资助机构	National Key Technology Research and Development Program of China(2015BAK45B01) ; National Natural Science Foundation of China(21476242) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/24027]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,et al. Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production[J]. WASTE AND BIOMASS VALORIZATION,2018,9(3):369-377.
APA	Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,Guo, Chen,&Liu, Chunzhao.(2018).Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production.WASTE AND BIOMASS VALORIZATION,9(3),369-377.
MLA	Samak, Nadia A.,et al."Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production".WASTE AND BIOMASS VALORIZATION 9.3(2018):369-377.