Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes | |
Yang, M.1; Mu, T.1; Zhong, W.2; Olajuyin, A. M.2; Xing, J.1 | |
刊名 | LETTERS IN APPLIED MICROBIOLOGY
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2017-08-01 | |
卷号 | 65期号:2页码:165-172 |
关键词 | Entner-doudoroff Pathway Genome-wide Transcriptome Gluconate Metabolism Pyruvate Redox State |
ISSN号 | 0266-8254 |
DOI | 10.1111/lam.12758 |
文献子类 | Article |
英文摘要 | For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome-wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner-Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate-producing strains. |
WOS关键词 | Carbon Source ; Glycolytic Flux ; Acid ; Dehydrogenase ; Enhancement ; Glucose ; Mutant ; Growth |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000406935400009 |
资助机构 | National High Technology Research and Development Program of China(2014AA021900) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/23147] ![]() |
专题 | 过程工程研究所_湿法冶金清洁生产技术国家工程实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China 2.Grad Sch Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, M.,Mu, T.,Zhong, W.,et al. Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes[J]. LETTERS IN APPLIED MICROBIOLOGY,2017,65(2):165-172. |
APA | Yang, M.,Mu, T.,Zhong, W.,Olajuyin, A. M.,&Xing, J..(2017).Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes.LETTERS IN APPLIED MICROBIOLOGY,65(2),165-172. |
MLA | Yang, M.,et al."Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes".LETTERS IN APPLIED MICROBIOLOGY 65.2(2017):165-172. |
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