Multi-Patterned Dynamics of Mitochondrial Fission and Fusion in a Living Cell

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	Multi-Patterned Dynamics of Mitochondrial Fission and Fusion in a Living Cell
	Wang, Shiqi 1,2,6; Xiao, Weiming 3,4,5; Shan, Sicong 1,2; Jiang, Chunsun 3; Chen, Ming 3; Zhang, Yan 1,2; Lu, Shouqin 1,2; Chen, Juan 1,2; Zhang, Chuanmao 4,5; Chen, Quan 3
刊名	PLOS ONE
	2012-05-23
卷号	7 期号:5
文献子类	Article
英文摘要	Mitochondria are highly-dynamic organelles, but it is challenging to monitor quantitatively their dynamics in a living cell. Here we developed a novel approach to determine the global occurrence of mitochondrial fission and fusion events in living human epithelial cells (Hela) and mouse embryonic fibroblast cells (MEF). Distinct patterns of sequential events including fusion followed by fission (Fu-Fi), the so-called "kiss and run'' model previously described, fission followed by fusion (Fi-Fu), fusion followed by fusion (Fu-Fu), and fission followed by fission (Fi-Fi) were observed concurrently. The paired events appeared in high frequencies with short lifetimes and large sizes of individual mitochondria, as compared to those for unpaired events. The high frequencies of paired events were found to be biologically significant. The presence of membrane uncoupler CCCP enhanced the frequency of paired events (from both Fu-Fi and Fi-Fu patterns) with a reduced mitochondrial size. Knock-out of mitofusin protein Mfn1 increased the frequency of fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. These results indicated that the paired events were dominant but unpaired events were not negligible, which provided a new insight into mitochondrial dynamics. In addition to kiss and run model of action, our data suggest that, from a global visualization over an entire cell, multiple patterns of action appeared in mitochondrial fusion and fission.
WOS关键词	MAMMALIAN-CELLS ; YEAST ; MICROTUBULES ; SEGREGATION ; MORPHOLOGY ; APOPTOSIS ; TRANSPORT ; MOVEMENT
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000305335800001
内容类型	期刊论文
源URL	[http://124.16.247.212/handle/311034/7211]
专题	古脊椎动物与古人类研究所_图书馆1
作者单位	1.Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Beijing, Peoples R China 4.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China 5.Peking Univ, Natl Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China 6.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shiqi,Xiao, Weiming,Shan, Sicong,et al. Multi-Patterned Dynamics of Mitochondrial Fission and Fusion in a Living Cell[J]. PLOS ONE,2012,7(5).
APA	Wang, Shiqi.,Xiao, Weiming.,Shan, Sicong.,Jiang, Chunsun.,Chen, Ming.,...&Long, Mian.(2012).Multi-Patterned Dynamics of Mitochondrial Fission and Fusion in a Living Cell.PLOS ONE,7(5).
MLA	Wang, Shiqi,et al."Multi-Patterned Dynamics of Mitochondrial Fission and Fusion in a Living Cell".PLOS ONE 7.5(2012).