QQ客服 官方微博 反馈留言

	TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro
	Zhou, Wenshan2; Liang, Hualei2; Xie, Ping1,2; Zhang, Xuezhen2; Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.
刊名	FRESENIUS ENVIRONMENTAL BULLETIN
	2012
卷号	21期号:8A页码:2274-2281
关键词	Erythrocytes Lipid Peroxidation Oxidative Stress In Vitro Haemolysis Microcystin-lr
ISSN号	1018-4619
文献子类	Article
英文摘要	Haematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.; Haematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.
WOS关键词	OXIDATIVE STRESS RESPONSES ; CULTURED RAT HEPATOCYTES ; LIPID-PEROXIDATION ; FREE-RADICALS ; DAMAGE ; EXPOSURE ; LIVER ; EXTRACTS ; ACETYLCHOLINESTERASE ; NA+,K+-ATPASE
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000309326100017
资助机构	National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160]
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17194]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Wenshan,Liang, Hualei,Xie, Ping,et al. TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(8A):2274-2281.
APA	Zhou, Wenshan,Liang, Hualei,Xie, Ping,Zhang, Xuezhen,&Xie, P .(2012).TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro.FRESENIUS ENVIRONMENTAL BULLETIN,21(8A),2274-2281.
MLA	Zhou, Wenshan,et al."TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro".FRESENIUS ENVIRONMENTAL BULLETIN 21.8A(2012):2274-2281.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。