Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp

doi:10.1016/j.aquatox.2012.03.004

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	Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp
	He, Jun 1; Chen, Jun 1; Xie, Ping 1; Zhang, Dawen 1,3; Li, Guangyu 1; Wu, Laiyan 1; Zhang, Wei 1; Guo, Xiaochun 2; Li, Shangchun 1; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China.
刊名	AQUATIC TOXICOLOGY
	2012-07-15
卷号	116 期号:-页码:61-68
关键词	Quantification Mclr-gsh/cys Mcrr-gsh/cys Bighead Carp
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2012.03.004
文献子类	Article
英文摘要	Glutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.; Glutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.
WOS关键词	TANDEM MASS-SPECTROMETRY ; PROTEIN PHOSPHATASE-1 ; INHIBITORY-ACTIVITY ; LR ; TOXICITY ; LIVER ; FISH ; RR ; IDENTIFICATION ; METABOLITES
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
WOS记录号	WOS:000304290900009
资助机构	National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077]
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16859]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者	Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 3.Jiang Xi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Peoples R China
推荐引用方式 GB/T 7714	He, Jun,Chen, Jun,Xie, Ping,et al. Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp[J]. AQUATIC TOXICOLOGY,2012,116(-):61-68.
APA	He, Jun.,Chen, Jun.,Xie, Ping.,Zhang, Dawen.,Li, Guangyu.,...&Chen, J .(2012).Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp.AQUATIC TOXICOLOGY,116(-),61-68.
MLA	He, Jun,et al."Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp".AQUATIC TOXICOLOGY 116.-(2012):61-68.