Enzymatic hydrogelation of self-assembling peptide I4K2 and its antibacterial and drug sustained-release activities

doi:10.1039/c7ra09743c

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 仿生能源与储能系统团队

	Enzymatic hydrogelation of self-assembling peptide I4K2 and its antibacterial and drug sustained-release activities
	Bai, Jingkun 1,2; Gong, Zhongying 1; Wang, Jingxin 2; Wang, Chengdong 3
刊名	RSC ADVANCES
	2017
卷号	7 期号:77 页码:48631-48638
DOI	10.1039/c7ra09743c
文献子类	Article
英文摘要	Hydrogels provide great potential for biomedical applications. For clinical use, hydrogels could be used as scaffold materials for cell culture, regenerative medicine and drugs release with bactericidal properties. The amphiphilic peptide I4K2 is designed to inhibit bacterial growth through membrane permeation mechanisms. I4K2 is found to be able to self-assemble into nanofibers and form hydrogels in the presence of an enzyme (plasma amine oxidase, PAO). HPLC and MALDI-TOF-MS data show that PAO promoted the oxidation of the 3-amine of the lysine side chain. The cross-linking of I4K2 molecules catalyzed by PAO leads to a decrease in the amount of the positive charge of the system, which enhances the interaction between the self-assembled nanofibers and contributes to the formation of hydrogels. This self-supported hydrogel showed antibacterial activity against both G(+) and G(-) bacteria and has low cytotoxicity, which enable it be consequently used as an antimicrobial agent or biological engineering scaffold material. The hydrogel also possesses good drug sustained-release activities. These advantages result in the great potential of this enzymatic I4K2 hydrogel for biomedical applications.
WOS关键词	PLASMA AMINE OXIDASE ; 3D CELL-CULTURE ; SUPRAMOLECULAR HYDROGELS ; LYSYL OXIDASE ; MOLECULAR-MECHANISMS ; DELIVERY VEHICLES ; CROSS-LINKING ; PLANT-TISSUE ; DESTABILIZATION ; AMPHIPHILES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000413777800010
资助机构	Weifang Medical University
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9736]
专题	青岛生物能源与过程研究所_仿生能源与储能系统团队
作者单位	1.Weifang Med Univ, Sch Biosci & Technol, Shandong Prov Key Lab Biopharmaceut, 7166 Baotong West St, Weifang 261042, Peoples R China 2.China Univ Petr East China, Ctr Bioengn & Biotechnol, 66 Changjiang West Rd, Qingdao 266580, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Ind Energy Storage Res Inst, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Bai, Jingkun,Gong, Zhongying,Wang, Jingxin,et al. Enzymatic hydrogelation of self-assembling peptide I4K2 and its antibacterial and drug sustained-release activities[J]. RSC ADVANCES,2017,7(77):48631-48638.
APA	Bai, Jingkun,Gong, Zhongying,Wang, Jingxin,&Wang, Chengdong.(2017).Enzymatic hydrogelation of self-assembling peptide I4K2 and its antibacterial and drug sustained-release activities.RSC ADVANCES,7(77),48631-48638.
MLA	Bai, Jingkun,et al."Enzymatic hydrogelation of self-assembling peptide I4K2 and its antibacterial and drug sustained-release activities".RSC ADVANCES 7.77(2017):48631-48638.