Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells | |
He, Yuehui1,2,3; Zhang, Peng1,2,3; Huang, Shi1,2,3; Wang, Tingting1,2,3; Ji, Yuetong1,2,3; Xu, Jian1,2,3,4 | |
刊名 | BIOTECHNOLOGY FOR BIOFUELS
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2017-11-17 | |
卷号 | 10 |
关键词 | Single-cell Raman Spectroscopy Starch Content Protein Content Triacylglycerol Content Phenotypic Heterogeneity Sampling Depth Chlamydomonas Reinhardtii Microalgae |
DOI | 10.1186/s13068-017-0967-x |
文献子类 | Article |
英文摘要 | Background: Current approaches for quantification of major energy-storage forms in microalgae, including starch, protein and lipids, generally require cell cultivation to collect biomass followed by tedious and time-consuming analytical procedures. Thus, label-free, non-destructive and simultaneous quantification of such macromolecules at single-cell resolution is highly desirable in microalgal feedstock development and bioprocess control. |
WOS关键词 | RAMAN-SPECTROSCOPY ; MICROBIAL-CELLS ; YEAST-CELLS ; HETEROGENEITY ; MICROSPECTROSCOPY ; LIVE ; POPULATIONS ; METABOLITES ; ROBUSTNESS ; MECHANISMS |
WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
语种 | 英语 |
WOS记录号 | WOS:000415578800001 |
资助机构 | NSFC(31327001 ; CAS(XDB15040100) ; China Postdoctoral Science Foundation Grant(2016LH00036) ; Shandong Province(ZR2014CQ005 ; State Development & Investment Corporation (SDIC) ; 31425002 ; 2015ZDJS04002) ; 31400089) |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/9696] ![]() |
专题 | 青岛生物能源与过程研究所_单细胞中心 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | He, Yuehui,Zhang, Peng,Huang, Shi,et al. Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells[J]. BIOTECHNOLOGY FOR BIOFUELS,2017,10. |
APA | He, Yuehui,Zhang, Peng,Huang, Shi,Wang, Tingting,Ji, Yuetong,&Xu, Jian.(2017).Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells.BIOTECHNOLOGY FOR BIOFUELS,10. |
MLA | He, Yuehui,et al."Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells".BIOTECHNOLOGY FOR BIOFUELS 10(2017). |
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