Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants

doi:10.1128/AEM.00039-18

	Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants
	Fu, Huihui 1,2,3; Liu, Lulu 1,2; Dong, Ziyang 1,2; Guo, Shupan 1,2; Gao, Haichun 1,2
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2018-04-01
卷号	84 期号:8
关键词	Iron Homeostasis Shewanella Fur Regulation Respiration Cytochrome c
ISSN号	0099-2240
DOI	10.1128/AEM.00039-18
文献子类	Article
英文摘要	Iron, a major protein cofactor, is essential for most organisms but can simultaneously be toxic. Iron homeostasis thus has to be effectively maintained under a range of iron regimes. This may be particularly true with Shewanella oneidensis, a representative of dissimilatory metal-reducing bacteria (DMRB), which are capable of respiring a variety of chemicals as electron acceptors (EAs), including iron ores. Although iron respiration and its regulation have been extensively studied in this bacterium, how iron homeostasis is maintained remains largely unknown. Here, we report that the loss of the iron homeostasis master regulator Fur negatively affects the respiration of all EAs tested. This defect appears mainly to be a result of reduced cytochrome c (cyt c) production, despite a decrease in the expression of reductases that are under the direct control of Fur. We also show that S. oneidensis Fur interacts with canonical Fur box motifs in F-F-x-R configuration rather than the palindromic motif proposed before. The fur mutant has lowered total iron and increased free iron contents. Under iron-rich conditions, overproduction of the major iron storage protein Bfr elevates the total iron levels of the fur mutant over those of the wildtype but does not affect free iron levels. Intriguingly, such an operation only marginally improves cyt c production by affecting heme b biosynthesis. It is established that iron dictates heme b/cyt c biosynthesis in S. oneidensis fur(+) strains, but the fur mutation annuls the dependence of heme b/cyt c biosynthesis on iron. Overall, our results suggest that Fur has a profound impact on the iron homeostasis of S. oneidensis, through which many physiological processes, especially respiration, are transformed.
WOS关键词	ANAEROBIC RESPIRATION ; OXIDATIVE STRESS ; ESCHERICHIA-COLI ; CYTOCHROME ; METABOLISM ; MR-1 ; MECHANISMS ; REGULATOR ; PROTEIN ; INVOLVEMENT
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000429665700001
资助机构	National Natural Science Foundation of China(41476105) ; Natural Science Foundation of Zhejiang Province(LZ17C010001)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10825]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Gao, Haichun
作者单位	1.Zhejiang Univ, Inst Microbiol, Hangzhou, Zhejiang, Peoples R China 2.Zhejiang Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Fu, Huihui,Liu, Lulu,Dong, Ziyang,et al. Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(8).
APA	Fu, Huihui,Liu, Lulu,Dong, Ziyang,Guo, Shupan,&Gao, Haichun.(2018).Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(8).
MLA	Fu, Huihui,et al."Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.8(2018).